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Calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons

The rodent vomeronasal organ plays a crucial role in several social behaviors. Detection of pheromones or other emitted signaling molecules occurs in the dendritic microvilli of vomeronasal sensory neurons, where the binding of molecules to vomeronasal receptors leads to the influx of sodium and cal...

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Autores principales: Dibattista, Michele, Amjad, Asma, Maurya, Devendra Kumar, Sagheddu, Claudia, Montani, Giorgia, Tirindelli, Roberto, Menini, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3382724/
https://www.ncbi.nlm.nih.gov/pubmed/22732308
http://dx.doi.org/10.1085/jgp.201210780
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author Dibattista, Michele
Amjad, Asma
Maurya, Devendra Kumar
Sagheddu, Claudia
Montani, Giorgia
Tirindelli, Roberto
Menini, Anna
author_facet Dibattista, Michele
Amjad, Asma
Maurya, Devendra Kumar
Sagheddu, Claudia
Montani, Giorgia
Tirindelli, Roberto
Menini, Anna
author_sort Dibattista, Michele
collection PubMed
description The rodent vomeronasal organ plays a crucial role in several social behaviors. Detection of pheromones or other emitted signaling molecules occurs in the dendritic microvilli of vomeronasal sensory neurons, where the binding of molecules to vomeronasal receptors leads to the influx of sodium and calcium ions mainly through the transient receptor potential canonical 2 (TRPC2) channel. To investigate the physiological role played by the increase in intracellular calcium concentration in the apical region of these neurons, we produced localized, rapid, and reproducible increases in calcium concentration with flash photolysis of caged calcium and measured calcium-activated currents with the whole cell voltage-clamp technique. On average, a large inward calcium-activated current of −261 pA was measured at −50 mV, rising with a time constant of 13 ms. Ion substitution experiments showed that this current is anion selective. Moreover, the chloride channel blockers niflumic acid and 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid partially inhibited the calcium-activated current. These results directly demonstrate that a large chloride current can be activated by calcium in the apical region of mouse vomeronasal sensory neurons. Furthermore, we showed by immunohistochemistry that the calcium-activated chloride channels TMEM16A/anoctamin1 and TMEM16B/anoctamin2 are present in the apical layer of the vomeronasal epithelium, where they largely colocalize with the TRPC2 transduction channel. Immunocytochemistry on isolated vomeronasal sensory neurons showed that TMEM16A and TMEM16B coexpress in the neuronal microvilli. Therefore, we conclude that microvilli of mouse vomeronasal sensory neurons have a high density of calcium-activated chloride channels that may play an important role in vomeronasal transduction.
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spelling pubmed-33827242013-01-01 Calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons Dibattista, Michele Amjad, Asma Maurya, Devendra Kumar Sagheddu, Claudia Montani, Giorgia Tirindelli, Roberto Menini, Anna J Gen Physiol Article The rodent vomeronasal organ plays a crucial role in several social behaviors. Detection of pheromones or other emitted signaling molecules occurs in the dendritic microvilli of vomeronasal sensory neurons, where the binding of molecules to vomeronasal receptors leads to the influx of sodium and calcium ions mainly through the transient receptor potential canonical 2 (TRPC2) channel. To investigate the physiological role played by the increase in intracellular calcium concentration in the apical region of these neurons, we produced localized, rapid, and reproducible increases in calcium concentration with flash photolysis of caged calcium and measured calcium-activated currents with the whole cell voltage-clamp technique. On average, a large inward calcium-activated current of −261 pA was measured at −50 mV, rising with a time constant of 13 ms. Ion substitution experiments showed that this current is anion selective. Moreover, the chloride channel blockers niflumic acid and 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid partially inhibited the calcium-activated current. These results directly demonstrate that a large chloride current can be activated by calcium in the apical region of mouse vomeronasal sensory neurons. Furthermore, we showed by immunohistochemistry that the calcium-activated chloride channels TMEM16A/anoctamin1 and TMEM16B/anoctamin2 are present in the apical layer of the vomeronasal epithelium, where they largely colocalize with the TRPC2 transduction channel. Immunocytochemistry on isolated vomeronasal sensory neurons showed that TMEM16A and TMEM16B coexpress in the neuronal microvilli. Therefore, we conclude that microvilli of mouse vomeronasal sensory neurons have a high density of calcium-activated chloride channels that may play an important role in vomeronasal transduction. The Rockefeller University Press 2012-07 /pmc/articles/PMC3382724/ /pubmed/22732308 http://dx.doi.org/10.1085/jgp.201210780 Text en © 2012 Dibattista et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Article
Dibattista, Michele
Amjad, Asma
Maurya, Devendra Kumar
Sagheddu, Claudia
Montani, Giorgia
Tirindelli, Roberto
Menini, Anna
Calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons
title Calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons
title_full Calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons
title_fullStr Calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons
title_full_unstemmed Calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons
title_short Calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons
title_sort calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3382724/
https://www.ncbi.nlm.nih.gov/pubmed/22732308
http://dx.doi.org/10.1085/jgp.201210780
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