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Rational Mutagenesis of Cyclodextrin Glucanotransferase at the Calcium Binding Regions for Enhancement of Thermostability

Studies related to the engineering of calcium binding sites of CGTase are limited. The calcium binding regions that are known for thermostability function were subjected to site-directed mutagenesis in this study. The starting gene-protein is a variant of CGTase Bacillus sp. G1, reported earlier and...

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Detalles Bibliográficos
Autores principales: Goh, Poh Hong, Illias, Rosli Md., Goh, Kian Mau
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3382795/
https://www.ncbi.nlm.nih.gov/pubmed/22754298
http://dx.doi.org/10.3390/ijms13055307

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