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Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703

Pokemon is an important proto-oncogene that plays a critical role in cellular oncogenic transformation and tumorigenesis. Anoikis, which is regulated by Bim-mediated apoptosis, is critical to cancer cell invasion and metastasis. We investigated the role of Pokemon in anoikis, and our results show th...

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Detalles Bibliográficos
Autores principales: Liu, Kun, Liu, Feng, Zhang, Nannan, Liu, Shiying, Jiang, Yuyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3382817/
https://www.ncbi.nlm.nih.gov/pubmed/22754333
http://dx.doi.org/10.3390/ijms13055818
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author Liu, Kun
Liu, Feng
Zhang, Nannan
Liu, Shiying
Jiang, Yuyang
author_facet Liu, Kun
Liu, Feng
Zhang, Nannan
Liu, Shiying
Jiang, Yuyang
author_sort Liu, Kun
collection PubMed
description Pokemon is an important proto-oncogene that plays a critical role in cellular oncogenic transformation and tumorigenesis. Anoikis, which is regulated by Bim-mediated apoptosis, is critical to cancer cell invasion and metastasis. We investigated the role of Pokemon in anoikis, and our results show that Pokemon renders liver cells resistant to anoikis via suppression of Bim transcription. We knocked-down Pokemon in human hepatoma cells QGY7703 with small interfering RNAs (siRNA). Knockdown of Pokemon alone did not significantly affect the growth and survival of QGY7703 cells but notably enhanced their sensitivity to apoptotic stress due to the presence of chemical agents or cell detachment, thereby inducing anoikis, as evidenced by flow cytometry and caspase-3 activity assays. In contrast, ectopic expression of Pokemon in HL7702 cells led to resistance to anoikis. Dual-luciferase reporter and ChIP assays illustrated that Pokemon suppressed Bim transcription via direct binding to its promoter. Our results suggest that Pokemon prevents anoikis through the suppression of Bim expression, which facilitates tumor cell invasion and metastasis. This Pokemon-Bim pathway may be an effective target for therapeutic intervention for cancer.
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spelling pubmed-33828172012-06-29 Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703 Liu, Kun Liu, Feng Zhang, Nannan Liu, Shiying Jiang, Yuyang Int J Mol Sci Article Pokemon is an important proto-oncogene that plays a critical role in cellular oncogenic transformation and tumorigenesis. Anoikis, which is regulated by Bim-mediated apoptosis, is critical to cancer cell invasion and metastasis. We investigated the role of Pokemon in anoikis, and our results show that Pokemon renders liver cells resistant to anoikis via suppression of Bim transcription. We knocked-down Pokemon in human hepatoma cells QGY7703 with small interfering RNAs (siRNA). Knockdown of Pokemon alone did not significantly affect the growth and survival of QGY7703 cells but notably enhanced their sensitivity to apoptotic stress due to the presence of chemical agents or cell detachment, thereby inducing anoikis, as evidenced by flow cytometry and caspase-3 activity assays. In contrast, ectopic expression of Pokemon in HL7702 cells led to resistance to anoikis. Dual-luciferase reporter and ChIP assays illustrated that Pokemon suppressed Bim transcription via direct binding to its promoter. Our results suggest that Pokemon prevents anoikis through the suppression of Bim expression, which facilitates tumor cell invasion and metastasis. This Pokemon-Bim pathway may be an effective target for therapeutic intervention for cancer. Molecular Diversity Preservation International (MDPI) 2012-05-15 /pmc/articles/PMC3382817/ /pubmed/22754333 http://dx.doi.org/10.3390/ijms13055818 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Liu, Kun
Liu, Feng
Zhang, Nannan
Liu, Shiying
Jiang, Yuyang
Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703
title Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703
title_full Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703
title_fullStr Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703
title_full_unstemmed Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703
title_short Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703
title_sort pokemon silencing leads to bim-mediated anoikis of human hepatoma cell qgy7703
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3382817/
https://www.ncbi.nlm.nih.gov/pubmed/22754333
http://dx.doi.org/10.3390/ijms13055818
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