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Application of HPLC for the Simultaneous Determination of Aceclofenac, Paracetamol and Tramadol Hydrochloride in Pharmaceutical Dosage Form

A simple, precise and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous estimation of aceclofenac (ACF), paracetamol (PCM) and tramadol hydrochloride (TRM) in pharmaceutical dosage form. The chromatographic separation was achieved on a HiQ-Sil™ HS C18 colu...

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Autores principales: Chandra, Preeti, Rathore, Atul Singh, Lohidasan, Sathiyanarayanan, Mahadik, Kakasaheb Ramoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Österreichische Apotheker-Verlagsgesellschaft 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383205/
https://www.ncbi.nlm.nih.gov/pubmed/22896821
http://dx.doi.org/10.3797/scipharm.1108-04
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author Chandra, Preeti
Rathore, Atul Singh
Lohidasan, Sathiyanarayanan
Mahadik, Kakasaheb Ramoo
author_facet Chandra, Preeti
Rathore, Atul Singh
Lohidasan, Sathiyanarayanan
Mahadik, Kakasaheb Ramoo
author_sort Chandra, Preeti
collection PubMed
description A simple, precise and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous estimation of aceclofenac (ACF), paracetamol (PCM) and tramadol hydrochloride (TRM) in pharmaceutical dosage form. The chromatographic separation was achieved on a HiQ-Sil™ HS C18 column (250×4.6 mm i.d., 5 μm particle size), kromatek analytical column at ambient temperature. The mobile phase consisted of 40: 60 (v/v); phosphate buffer (pH 6.0): methanol. The flow rate was set to 1.0 mL min(−1) and UV detection was carried out at 270 nm. The retention time (t(R)) for ACF, PCM and TRM were found to be 14.567 ± 0.02, 3.133 ± 0.01 and 7.858 ± 0.02 min, respectively. The validation of the proposed method was carried out for linearity, precision, robustness, limit of detection, limit of quantitation, speci city, accuracy and system suitability. The linear dynamic ranges were from 40–160 μg mL(−1) for ACF, 130–520 μg mL(−1) for PCM and 15–60 μg mL(−1) for TRM. The developed method can be used for routine quality control analysis of titled drugs in pharmaceutical dosage form.
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spelling pubmed-33832052012-08-15 Application of HPLC for the Simultaneous Determination of Aceclofenac, Paracetamol and Tramadol Hydrochloride in Pharmaceutical Dosage Form Chandra, Preeti Rathore, Atul Singh Lohidasan, Sathiyanarayanan Mahadik, Kakasaheb Ramoo Sci Pharm Research Article A simple, precise and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous estimation of aceclofenac (ACF), paracetamol (PCM) and tramadol hydrochloride (TRM) in pharmaceutical dosage form. The chromatographic separation was achieved on a HiQ-Sil™ HS C18 column (250×4.6 mm i.d., 5 μm particle size), kromatek analytical column at ambient temperature. The mobile phase consisted of 40: 60 (v/v); phosphate buffer (pH 6.0): methanol. The flow rate was set to 1.0 mL min(−1) and UV detection was carried out at 270 nm. The retention time (t(R)) for ACF, PCM and TRM were found to be 14.567 ± 0.02, 3.133 ± 0.01 and 7.858 ± 0.02 min, respectively. The validation of the proposed method was carried out for linearity, precision, robustness, limit of detection, limit of quantitation, speci city, accuracy and system suitability. The linear dynamic ranges were from 40–160 μg mL(−1) for ACF, 130–520 μg mL(−1) for PCM and 15–60 μg mL(−1) for TRM. The developed method can be used for routine quality control analysis of titled drugs in pharmaceutical dosage form. Österreichische Apotheker-Verlagsgesellschaft 2012 2012-01-31 /pmc/articles/PMC3383205/ /pubmed/22896821 http://dx.doi.org/10.3797/scipharm.1108-04 Text en © Chandra et al.; licensee Österreichische Apotheker-Verlagsgesellschaft m. b. H., Vienna, Austria. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chandra, Preeti
Rathore, Atul Singh
Lohidasan, Sathiyanarayanan
Mahadik, Kakasaheb Ramoo
Application of HPLC for the Simultaneous Determination of Aceclofenac, Paracetamol and Tramadol Hydrochloride in Pharmaceutical Dosage Form
title Application of HPLC for the Simultaneous Determination of Aceclofenac, Paracetamol and Tramadol Hydrochloride in Pharmaceutical Dosage Form
title_full Application of HPLC for the Simultaneous Determination of Aceclofenac, Paracetamol and Tramadol Hydrochloride in Pharmaceutical Dosage Form
title_fullStr Application of HPLC for the Simultaneous Determination of Aceclofenac, Paracetamol and Tramadol Hydrochloride in Pharmaceutical Dosage Form
title_full_unstemmed Application of HPLC for the Simultaneous Determination of Aceclofenac, Paracetamol and Tramadol Hydrochloride in Pharmaceutical Dosage Form
title_short Application of HPLC for the Simultaneous Determination of Aceclofenac, Paracetamol and Tramadol Hydrochloride in Pharmaceutical Dosage Form
title_sort application of hplc for the simultaneous determination of aceclofenac, paracetamol and tramadol hydrochloride in pharmaceutical dosage form
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383205/
https://www.ncbi.nlm.nih.gov/pubmed/22896821
http://dx.doi.org/10.3797/scipharm.1108-04
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