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A New Method for Distinguishing Colony Social Forms of the Fire Ant, Solenopsis invicta
Two distinct forms of colony social organization occur in the fire ant Solenopsis invicta Buren (Hymenoptera: Formicidae): colonies of the monogyne social form are headed by a single egg-laying queen, whereas those of the polygyne social form contain multiple egg-laying queens. This major difference...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
University of Wisconsin Library
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383434/ https://www.ncbi.nlm.nih.gov/pubmed/20673191 http://dx.doi.org/10.1673/031.010.7301 |
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author | Shoemaker, DeWayne Ascunce, Marina S. |
author_facet | Shoemaker, DeWayne Ascunce, Marina S. |
author_sort | Shoemaker, DeWayne |
collection | PubMed |
description | Two distinct forms of colony social organization occur in the fire ant Solenopsis invicta Buren (Hymenoptera: Formicidae): colonies of the monogyne social form are headed by a single egg-laying queen, whereas those of the polygyne social form contain multiple egg-laying queens. This major difference in social organization is associated with genetic variation at a single gene (Gp-9) whereby all polygyne queens possess at least one b-like allele, while monogyne queens lack such b-like alleles and instead harbor B-like alleles only. Further, a recent study of native populations revealed that all b-like alleles in polygyne queens consistently contain three diagnostic amino acid residues: possession of only one or two of these critical residues is not sufficient for polygyny. TaqMan® allelic discrimination assays were developed to survey the variable nucleotide sites associated with these three critical amino acid residues. The assays were validated by surveying nests of known social form from the species' introduced in the USA and from native South American ranges, as well as by comparing the results to Gp-9 sequence data from a subset of samples. The results demonstrate these new molecular assays consistently and accurately identify the variable nucleotides at all three sites characteristic of the B-like and b-like Gp-9 allele classes, allowing for accurate determination of colony social form. |
format | Online Article Text |
id | pubmed-3383434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | University of Wisconsin Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-33834342012-06-28 A New Method for Distinguishing Colony Social Forms of the Fire Ant, Solenopsis invicta Shoemaker, DeWayne Ascunce, Marina S. J Insect Sci Article Two distinct forms of colony social organization occur in the fire ant Solenopsis invicta Buren (Hymenoptera: Formicidae): colonies of the monogyne social form are headed by a single egg-laying queen, whereas those of the polygyne social form contain multiple egg-laying queens. This major difference in social organization is associated with genetic variation at a single gene (Gp-9) whereby all polygyne queens possess at least one b-like allele, while monogyne queens lack such b-like alleles and instead harbor B-like alleles only. Further, a recent study of native populations revealed that all b-like alleles in polygyne queens consistently contain three diagnostic amino acid residues: possession of only one or two of these critical residues is not sufficient for polygyny. TaqMan® allelic discrimination assays were developed to survey the variable nucleotide sites associated with these three critical amino acid residues. The assays were validated by surveying nests of known social form from the species' introduced in the USA and from native South American ranges, as well as by comparing the results to Gp-9 sequence data from a subset of samples. The results demonstrate these new molecular assays consistently and accurately identify the variable nucleotides at all three sites characteristic of the B-like and b-like Gp-9 allele classes, allowing for accurate determination of colony social form. University of Wisconsin Library 2010-06-22 /pmc/articles/PMC3383434/ /pubmed/20673191 http://dx.doi.org/10.1673/031.010.7301 Text en © 2010 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Shoemaker, DeWayne Ascunce, Marina S. A New Method for Distinguishing Colony Social Forms of the Fire Ant, Solenopsis invicta |
title | A New Method for Distinguishing Colony Social Forms of the Fire Ant, Solenopsis invicta
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title_full | A New Method for Distinguishing Colony Social Forms of the Fire Ant, Solenopsis invicta
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title_fullStr | A New Method for Distinguishing Colony Social Forms of the Fire Ant, Solenopsis invicta
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title_full_unstemmed | A New Method for Distinguishing Colony Social Forms of the Fire Ant, Solenopsis invicta
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title_short | A New Method for Distinguishing Colony Social Forms of the Fire Ant, Solenopsis invicta
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title_sort | new method for distinguishing colony social forms of the fire ant, solenopsis invicta |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383434/ https://www.ncbi.nlm.nih.gov/pubmed/20673191 http://dx.doi.org/10.1673/031.010.7301 |
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