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Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation
The generation of affinity reagents to large numbers of human proteins depends on the ability to express the target proteins as high-quality antigens. The Structural Genomics Consortium (SGC) focuses on the production and structure determination of human proteins. In a 7-year period, the SGC has dep...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383991/ https://www.ncbi.nlm.nih.gov/pubmed/22027370 http://dx.doi.org/10.1016/j.nbt.2011.10.007 |