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Shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation

BACKGROUND: The spectrin cytoskeleton is emerging as an important host cell target of enteric bacterial pathogens. Recent studies have identified a crucial role for spectrin and its associated proteins during key pathogenic processes of Listeria monocytogenes and Salmonella Typhimurium infections. H...

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Autores principales: Ruetz, Tyson J, Lin, Ann E, Guttman, Julian A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384245/
https://www.ncbi.nlm.nih.gov/pubmed/22424399
http://dx.doi.org/10.1186/1471-2180-12-36
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author Ruetz, Tyson J
Lin, Ann E
Guttman, Julian A
author_facet Ruetz, Tyson J
Lin, Ann E
Guttman, Julian A
author_sort Ruetz, Tyson J
collection PubMed
description BACKGROUND: The spectrin cytoskeleton is emerging as an important host cell target of enteric bacterial pathogens. Recent studies have identified a crucial role for spectrin and its associated proteins during key pathogenic processes of Listeria monocytogenes and Salmonella Typhimurium infections. Here we investigate the involvement of spectrin cytoskeletal components during the pathogenesis of the invasive pathogen Shigella flexneri. RESULTS: Immunofluorescent microscopy reveals that protein 4.1 (p4.1), but not adducin or spectrin, is robustly recruited to sites of S. flexneri membrane ruffling during epithelial cell invasion. Through siRNA-mediated knockdowns, we identify an important role for spectrin and the associated proteins adducin and p4.1 during S. flexneri invasion. Following internalization, all three proteins are recruited to the internalized bacteria, however upon generation of actin-rich comet tails, we observed spectrin recruitment to those structures in the absence of adducin or p4.1. CONCLUSION: These findings highlight the importance of the spectrin cytoskeletal network during S. flexneri pathogenesis and further demonstrate that pathogenic events that were once thought to exclusively recruit the actin cytoskeletal system require additional cytoskeletal networks.
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spelling pubmed-33842452012-06-28 Shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation Ruetz, Tyson J Lin, Ann E Guttman, Julian A BMC Microbiol Research Article BACKGROUND: The spectrin cytoskeleton is emerging as an important host cell target of enteric bacterial pathogens. Recent studies have identified a crucial role for spectrin and its associated proteins during key pathogenic processes of Listeria monocytogenes and Salmonella Typhimurium infections. Here we investigate the involvement of spectrin cytoskeletal components during the pathogenesis of the invasive pathogen Shigella flexneri. RESULTS: Immunofluorescent microscopy reveals that protein 4.1 (p4.1), but not adducin or spectrin, is robustly recruited to sites of S. flexneri membrane ruffling during epithelial cell invasion. Through siRNA-mediated knockdowns, we identify an important role for spectrin and the associated proteins adducin and p4.1 during S. flexneri invasion. Following internalization, all three proteins are recruited to the internalized bacteria, however upon generation of actin-rich comet tails, we observed spectrin recruitment to those structures in the absence of adducin or p4.1. CONCLUSION: These findings highlight the importance of the spectrin cytoskeletal network during S. flexneri pathogenesis and further demonstrate that pathogenic events that were once thought to exclusively recruit the actin cytoskeletal system require additional cytoskeletal networks. BioMed Central 2012-03-16 /pmc/articles/PMC3384245/ /pubmed/22424399 http://dx.doi.org/10.1186/1471-2180-12-36 Text en Copyright ©2012 Ruetz et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ruetz, Tyson J
Lin, Ann E
Guttman, Julian A
Shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation
title Shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation
title_full Shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation
title_fullStr Shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation
title_full_unstemmed Shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation
title_short Shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation
title_sort shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384245/
https://www.ncbi.nlm.nih.gov/pubmed/22424399
http://dx.doi.org/10.1186/1471-2180-12-36
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