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Real-time assay for testing components of protein synthesis

We present a flexible, real-time-coupled transcription–translation assay that involves the continuous monitoring of fluorescent Emerald GFP formation. Along with numerical simulation of a reaction kinetics model, the assay permits quantitative estimation of the effects on full-length protein synthes...

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Detalles Bibliográficos
Autores principales:	Rosenblum, Gabriel, Chen, Chunlai, Kaur, Jaskiran, Cui, Xiaonan, Goldman, Yale E., Cooperman, Barry S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2012
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384345/ https://www.ncbi.nlm.nih.gov/pubmed/22422844 http://dx.doi.org/10.1093/nar/gks232

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