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Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis

During vegetative growth, Saccharomyces cerevisiae cells divide asymmetrically: the mother cell buds to produce a smaller daughter cell. This daughter asymmetrically inherits the transcription factor Ace2, which activates daughter-specific transcriptional programs. In this paper, we investigate when...

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Autores principales: Boettcher, Barbara, Marquez-Lago, Tatiana T., Bayer, Mathias, Weiss, Eric L., Barral, Yves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384416/
https://www.ncbi.nlm.nih.gov/pubmed/22711697
http://dx.doi.org/10.1083/jcb.201112117
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author Boettcher, Barbara
Marquez-Lago, Tatiana T.
Bayer, Mathias
Weiss, Eric L.
Barral, Yves
author_facet Boettcher, Barbara
Marquez-Lago, Tatiana T.
Bayer, Mathias
Weiss, Eric L.
Barral, Yves
author_sort Boettcher, Barbara
collection PubMed
description During vegetative growth, Saccharomyces cerevisiae cells divide asymmetrically: the mother cell buds to produce a smaller daughter cell. This daughter asymmetrically inherits the transcription factor Ace2, which activates daughter-specific transcriptional programs. In this paper, we investigate when and how this asymmetry is established and maintained. We show that Ace2 asymmetry is initiated in the elongated, but undivided, anaphase nucleus. At this stage, the nucleoplasm was highly compartmentalized; little exchange was observed for nucleoplasmic proteins between mother and bud. Using photobleaching and in silico modeling, we show that diffusion barriers compartmentalize the nuclear membranes. In contrast, the behavior of proteins in the nucleoplasm is well explained by the dumbbell shape of the anaphase nucleus. This compartmentalization of the nucleoplasm promoted Ace2 asymmetry in anaphase nuclei. Thus, our data indicate that yeast cells use the process of closed mitosis and the morphological constraints associated with it to asymmetrically segregate nucleoplasmic components.
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spelling pubmed-33844162012-12-25 Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis Boettcher, Barbara Marquez-Lago, Tatiana T. Bayer, Mathias Weiss, Eric L. Barral, Yves J Cell Biol Research Articles During vegetative growth, Saccharomyces cerevisiae cells divide asymmetrically: the mother cell buds to produce a smaller daughter cell. This daughter asymmetrically inherits the transcription factor Ace2, which activates daughter-specific transcriptional programs. In this paper, we investigate when and how this asymmetry is established and maintained. We show that Ace2 asymmetry is initiated in the elongated, but undivided, anaphase nucleus. At this stage, the nucleoplasm was highly compartmentalized; little exchange was observed for nucleoplasmic proteins between mother and bud. Using photobleaching and in silico modeling, we show that diffusion barriers compartmentalize the nuclear membranes. In contrast, the behavior of proteins in the nucleoplasm is well explained by the dumbbell shape of the anaphase nucleus. This compartmentalization of the nucleoplasm promoted Ace2 asymmetry in anaphase nuclei. Thus, our data indicate that yeast cells use the process of closed mitosis and the morphological constraints associated with it to asymmetrically segregate nucleoplasmic components. The Rockefeller University Press 2012-06-25 /pmc/articles/PMC3384416/ /pubmed/22711697 http://dx.doi.org/10.1083/jcb.201112117 Text en © 2012 Boettcher et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Boettcher, Barbara
Marquez-Lago, Tatiana T.
Bayer, Mathias
Weiss, Eric L.
Barral, Yves
Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis
title Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis
title_full Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis
title_fullStr Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis
title_full_unstemmed Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis
title_short Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis
title_sort nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384416/
https://www.ncbi.nlm.nih.gov/pubmed/22711697
http://dx.doi.org/10.1083/jcb.201112117
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