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Functional Importance of the DNA Binding Activity of Candida albicans Czf1p
The human opportunistic pathogen Candida albicans undergoes a reversible morphological transition between the yeast and hyphal states in response to a variety of signals. One such environmental trigger is growth within a semisolid matrix such as agar medium. This growth condition is of interest beca...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384613/ https://www.ncbi.nlm.nih.gov/pubmed/22761849 http://dx.doi.org/10.1371/journal.pone.0039624 |
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author | Petrovska, Ivana Kumamoto, Carol A. |
author_facet | Petrovska, Ivana Kumamoto, Carol A. |
author_sort | Petrovska, Ivana |
collection | PubMed |
description | The human opportunistic pathogen Candida albicans undergoes a reversible morphological transition between the yeast and hyphal states in response to a variety of signals. One such environmental trigger is growth within a semisolid matrix such as agar medium. This growth condition is of interest because it may mimic the growth of C. albicans in contact with host tissue during infection. During growth within a semisolid matrix, hyphal growth is positively regulated by the transcriptional regulator Czf1p and negatively by a second key transcriptional regulator, Efg1p. Genetic studies indicate that Czf1p, a member of the zinc-cluster family of transcriptional regulators, exerts its function by opposing the inhibitory influence of Efg1p on matrix-induced filamentous growth. We examined the importance of the two known activities of Czf1p, DNA-binding and interaction with Efg1p. We found that the two activities were separable by mutation allowing us to demonstrate that the DNA-binding activity of Czf1p was essential for its role as a positive regulator of morphogenesis. Surprisingly, however, interactions with Efg1p appeared to be largely dispensable. Our studies provide the first evidence of a key role for the DNA-binding activity of Czf1p in the morphological yeast-to-hyphal transition triggered by matrix-embedded growth. |
format | Online Article Text |
id | pubmed-3384613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33846132012-07-03 Functional Importance of the DNA Binding Activity of Candida albicans Czf1p Petrovska, Ivana Kumamoto, Carol A. PLoS One Research Article The human opportunistic pathogen Candida albicans undergoes a reversible morphological transition between the yeast and hyphal states in response to a variety of signals. One such environmental trigger is growth within a semisolid matrix such as agar medium. This growth condition is of interest because it may mimic the growth of C. albicans in contact with host tissue during infection. During growth within a semisolid matrix, hyphal growth is positively regulated by the transcriptional regulator Czf1p and negatively by a second key transcriptional regulator, Efg1p. Genetic studies indicate that Czf1p, a member of the zinc-cluster family of transcriptional regulators, exerts its function by opposing the inhibitory influence of Efg1p on matrix-induced filamentous growth. We examined the importance of the two known activities of Czf1p, DNA-binding and interaction with Efg1p. We found that the two activities were separable by mutation allowing us to demonstrate that the DNA-binding activity of Czf1p was essential for its role as a positive regulator of morphogenesis. Surprisingly, however, interactions with Efg1p appeared to be largely dispensable. Our studies provide the first evidence of a key role for the DNA-binding activity of Czf1p in the morphological yeast-to-hyphal transition triggered by matrix-embedded growth. Public Library of Science 2012-06-27 /pmc/articles/PMC3384613/ /pubmed/22761849 http://dx.doi.org/10.1371/journal.pone.0039624 Text en Petrovska, Kumamoto. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Petrovska, Ivana Kumamoto, Carol A. Functional Importance of the DNA Binding Activity of Candida albicans Czf1p |
title | Functional Importance of the DNA Binding Activity of Candida albicans Czf1p |
title_full | Functional Importance of the DNA Binding Activity of Candida albicans Czf1p |
title_fullStr | Functional Importance of the DNA Binding Activity of Candida albicans Czf1p |
title_full_unstemmed | Functional Importance of the DNA Binding Activity of Candida albicans Czf1p |
title_short | Functional Importance of the DNA Binding Activity of Candida albicans Czf1p |
title_sort | functional importance of the dna binding activity of candida albicans czf1p |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384613/ https://www.ncbi.nlm.nih.gov/pubmed/22761849 http://dx.doi.org/10.1371/journal.pone.0039624 |
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