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Detection of Ligation Products of DNA Linkers with 5′-OH Ends by Denaturing PAGE Silver Stain
To explore if DNA linkers with 5′-hydroxyl (OH) ends could be joined by commercial T4 and E. coli DNA ligase, these linkers were synthesized by using the solid-phase phosphoramidite method and joined by using commercial T4 and E. coli DNA ligases. The ligation products were detected by using denatur...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384673/ https://www.ncbi.nlm.nih.gov/pubmed/22761747 http://dx.doi.org/10.1371/journal.pone.0039251 |