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In Vivo Visualization of Heterogeneous Intratumoral Distribution of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests
Purpose. We aimed to clearly visualize heterogeneous distribution of hypoxia-inducible factor 1α (HIF) activity in tumor tissues in vivo. Methods. We synthesized of (125)I-IPOS, a (125)I labeled chimeric protein probe, that would visualize HIF activity. The biodistribution of (125)I-IPOS in FM3A tum...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385015/ https://www.ncbi.nlm.nih.gov/pubmed/22778544 http://dx.doi.org/10.1155/2012/262741 |
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author | Fujii, Hirofumi Yamaguchi, Masayuki Inoue, Kazumasa Mutou, Yasuko Ueda, Masashi Saji, Hideo Kizaka-Kondoh, Shinae Moriyama, Noriyuki Umeda, Izumi O. |
author_facet | Fujii, Hirofumi Yamaguchi, Masayuki Inoue, Kazumasa Mutou, Yasuko Ueda, Masashi Saji, Hideo Kizaka-Kondoh, Shinae Moriyama, Noriyuki Umeda, Izumi O. |
author_sort | Fujii, Hirofumi |
collection | PubMed |
description | Purpose. We aimed to clearly visualize heterogeneous distribution of hypoxia-inducible factor 1α (HIF) activity in tumor tissues in vivo. Methods. We synthesized of (125)I-IPOS, a (125)I labeled chimeric protein probe, that would visualize HIF activity. The biodistribution of (125)I-IPOS in FM3A tumor-bearing mice was evaluated. Then, the intratumoral localization of this probe was observed by autoradiography, and it was compared with histopathological findings. The distribution of (125)I-IPOS in tumors was imaged by a small animal SPECT/CT scanner. The obtained in vivo SPECT-CT fusion images were compared with ex vivo images of excised tumors. Fusion imaging with MRI was also examined. Results. (125)I-IPOS well accumulated in FM3A tumors. The intratumoral distribution of (125)I-IPOS by autoradiography was quite heterogeneous, and it partially overlapped with that of pimonidazole. High-resolution SPECT-CT fusion images successfully demonstrated the heterogeneity of (125)I-IPOS distribution inside tumors. SPECT-MRI fusion images could give more detailed information about the intratumoral distribution of (125)I-IPOS. Conclusion. High-resolution SPECT images successfully demonstrated heterogeneous intratumoral distribution of (125)I-IPOS. SPECT-CT fusion images, more favorably SPECT-MRI fusion images, would be useful to understand the features of heterogeneous intratumoral expression of HIF activity in vivo. |
format | Online Article Text |
id | pubmed-3385015 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33850152012-07-09 In Vivo Visualization of Heterogeneous Intratumoral Distribution of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests Fujii, Hirofumi Yamaguchi, Masayuki Inoue, Kazumasa Mutou, Yasuko Ueda, Masashi Saji, Hideo Kizaka-Kondoh, Shinae Moriyama, Noriyuki Umeda, Izumi O. J Biomed Biotechnol Research Article Purpose. We aimed to clearly visualize heterogeneous distribution of hypoxia-inducible factor 1α (HIF) activity in tumor tissues in vivo. Methods. We synthesized of (125)I-IPOS, a (125)I labeled chimeric protein probe, that would visualize HIF activity. The biodistribution of (125)I-IPOS in FM3A tumor-bearing mice was evaluated. Then, the intratumoral localization of this probe was observed by autoradiography, and it was compared with histopathological findings. The distribution of (125)I-IPOS in tumors was imaged by a small animal SPECT/CT scanner. The obtained in vivo SPECT-CT fusion images were compared with ex vivo images of excised tumors. Fusion imaging with MRI was also examined. Results. (125)I-IPOS well accumulated in FM3A tumors. The intratumoral distribution of (125)I-IPOS by autoradiography was quite heterogeneous, and it partially overlapped with that of pimonidazole. High-resolution SPECT-CT fusion images successfully demonstrated the heterogeneity of (125)I-IPOS distribution inside tumors. SPECT-MRI fusion images could give more detailed information about the intratumoral distribution of (125)I-IPOS. Conclusion. High-resolution SPECT images successfully demonstrated heterogeneous intratumoral distribution of (125)I-IPOS. SPECT-CT fusion images, more favorably SPECT-MRI fusion images, would be useful to understand the features of heterogeneous intratumoral expression of HIF activity in vivo. Hindawi Publishing Corporation 2012 2012-06-12 /pmc/articles/PMC3385015/ /pubmed/22778544 http://dx.doi.org/10.1155/2012/262741 Text en Copyright © 2012 Hirofumi Fujii et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Fujii, Hirofumi Yamaguchi, Masayuki Inoue, Kazumasa Mutou, Yasuko Ueda, Masashi Saji, Hideo Kizaka-Kondoh, Shinae Moriyama, Noriyuki Umeda, Izumi O. In Vivo Visualization of Heterogeneous Intratumoral Distribution of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests |
title |
In Vivo Visualization of Heterogeneous Intratumoral Distribution
of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests |
title_full |
In Vivo Visualization of Heterogeneous Intratumoral Distribution
of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests |
title_fullStr |
In Vivo Visualization of Heterogeneous Intratumoral Distribution
of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests |
title_full_unstemmed |
In Vivo Visualization of Heterogeneous Intratumoral Distribution
of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests |
title_short |
In Vivo Visualization of Heterogeneous Intratumoral Distribution
of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests |
title_sort | in vivo visualization of heterogeneous intratumoral distribution
of hypoxia-inducible factor-1α activity by the fusion of high-resolution spect and morphological imaging tests |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385015/ https://www.ncbi.nlm.nih.gov/pubmed/22778544 http://dx.doi.org/10.1155/2012/262741 |
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