Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris

The cDNA of endo-1,4-β-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of...

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Autores principales: Huy, Nguyen Duc, Thiyagarajan, Saravanakumar, Son, Yu-Lim, Park, Seung-Moon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2011
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385102/
https://www.ncbi.nlm.nih.gov/pubmed/22783089
http://dx.doi.org/10.4489/MYCO.2011.39.2.121
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author Huy, Nguyen Duc
Thiyagarajan, Saravanakumar
Son, Yu-Lim
Park, Seung-Moon
author_facet Huy, Nguyen Duc
Thiyagarajan, Saravanakumar
Son, Yu-Lim
Park, Seung-Moon
author_sort Huy, Nguyen Duc
collection PubMed
description The cDNA of endo-1,4-β-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.
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spelling pubmed-33851022012-07-10 Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris Huy, Nguyen Duc Thiyagarajan, Saravanakumar Son, Yu-Lim Park, Seung-Moon Mycobiology Research Note The cDNA of endo-1,4-β-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively. The Korean Society of Mycology 2011-06 2011-06-16 /pmc/articles/PMC3385102/ /pubmed/22783089 http://dx.doi.org/10.4489/MYCO.2011.39.2.121 Text en © The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Note
Huy, Nguyen Duc
Thiyagarajan, Saravanakumar
Son, Yu-Lim
Park, Seung-Moon
Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris
title Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris
title_full Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris
title_fullStr Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris
title_full_unstemmed Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris
title_short Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris
title_sort heterologous expression of endo-1,4-beta-xylanasea from phanerochaete chrysosporium in pichia pastoris
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385102/
https://www.ncbi.nlm.nih.gov/pubmed/22783089
http://dx.doi.org/10.4489/MYCO.2011.39.2.121
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