Trichoderma asperellum Chi42 Genes Encode Chitinase

Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in aga...

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Detalles Bibliográficos
Autores principales: Loc, Nguyen Hoang, Quang, Hoang Tan, Hung, Nguyen Bao, Huy, Nguyen Duc, Phuong, Truong Thi Bich, Ha, Tran Thi Thu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385114/
https://www.ncbi.nlm.nih.gov/pubmed/22783101
http://dx.doi.org/10.5941/MYCO.2011.39.3.182
Descripción
Sumario:Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.

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