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Breeding of New Strains of Mushroom by Basidiospore Chemical Mutagenesis

Chemical mutagenesis of basidiospores of Hypsizygus marmoreus generated new mushroom strains. The basidospores were treated with methanesulfonate methylester, an alkylating agent, to yield 400 mutant monokaryotic mycelia. Twenty fast-growing mycelia were selected and mated each other by hyphal fusio...

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Autores principales: Lee, Jia, Kang, Hyeon-Woo, Kim, Sang-Woo, Lee, Chang-Yun, Ro, Hyeon-Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385130/
https://www.ncbi.nlm.nih.gov/pubmed/22783115
http://dx.doi.org/10.5941/MYCO.2011.39.4.272
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author Lee, Jia
Kang, Hyeon-Woo
Kim, Sang-Woo
Lee, Chang-Yun
Ro, Hyeon-Su
author_facet Lee, Jia
Kang, Hyeon-Woo
Kim, Sang-Woo
Lee, Chang-Yun
Ro, Hyeon-Su
author_sort Lee, Jia
collection PubMed
description Chemical mutagenesis of basidiospores of Hypsizygus marmoreus generated new mushroom strains. The basidospores were treated with methanesulfonate methylester, an alkylating agent, to yield 400 mutant monokaryotic mycelia. Twenty fast-growing mycelia were selected and mated each other by hyphal fusion. Fifty out of the 190 matings were successful (mating rate of 26.3%), judged by the formation of clamp connections. The mutant dikaryons were cultivated to investigate their morphological and cultivation characteristics. Mutant strains No. 3 and No. 5 showed 10% and 6% increase in fruiting body production, respectively. Eight mutant strains showed delayed and reduced primordia formation, resulting in the reduced production yield with prolonged cultivation period. The number of the fruiting bodies of mutant No. 31, which displayed reduced primordial formation, was only 15, compared to the parental number of 65. Another interesting phenotype was a fruiting body with a flattened stipe and pileus. Dikaryons generated by mating with the mutant spore No. 14 produced flat fruiting bodies. Further molecular biological studies will provide details of the mechanism. This work shows that the chemical mutagenesis approach is highly utilizable in the development of mushroom strains as well as in the generation of resources for molecular genetic studies.
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spelling pubmed-33851302012-07-10 Breeding of New Strains of Mushroom by Basidiospore Chemical Mutagenesis Lee, Jia Kang, Hyeon-Woo Kim, Sang-Woo Lee, Chang-Yun Ro, Hyeon-Su Mycobiology Research Article Chemical mutagenesis of basidiospores of Hypsizygus marmoreus generated new mushroom strains. The basidospores were treated with methanesulfonate methylester, an alkylating agent, to yield 400 mutant monokaryotic mycelia. Twenty fast-growing mycelia were selected and mated each other by hyphal fusion. Fifty out of the 190 matings were successful (mating rate of 26.3%), judged by the formation of clamp connections. The mutant dikaryons were cultivated to investigate their morphological and cultivation characteristics. Mutant strains No. 3 and No. 5 showed 10% and 6% increase in fruiting body production, respectively. Eight mutant strains showed delayed and reduced primordia formation, resulting in the reduced production yield with prolonged cultivation period. The number of the fruiting bodies of mutant No. 31, which displayed reduced primordial formation, was only 15, compared to the parental number of 65. Another interesting phenotype was a fruiting body with a flattened stipe and pileus. Dikaryons generated by mating with the mutant spore No. 14 produced flat fruiting bodies. Further molecular biological studies will provide details of the mechanism. This work shows that the chemical mutagenesis approach is highly utilizable in the development of mushroom strains as well as in the generation of resources for molecular genetic studies. The Korean Society of Mycology 2011-12 2011-12-07 /pmc/articles/PMC3385130/ /pubmed/22783115 http://dx.doi.org/10.5941/MYCO.2011.39.4.272 Text en © The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lee, Jia
Kang, Hyeon-Woo
Kim, Sang-Woo
Lee, Chang-Yun
Ro, Hyeon-Su
Breeding of New Strains of Mushroom by Basidiospore Chemical Mutagenesis
title Breeding of New Strains of Mushroom by Basidiospore Chemical Mutagenesis
title_full Breeding of New Strains of Mushroom by Basidiospore Chemical Mutagenesis
title_fullStr Breeding of New Strains of Mushroom by Basidiospore Chemical Mutagenesis
title_full_unstemmed Breeding of New Strains of Mushroom by Basidiospore Chemical Mutagenesis
title_short Breeding of New Strains of Mushroom by Basidiospore Chemical Mutagenesis
title_sort breeding of new strains of mushroom by basidiospore chemical mutagenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385130/
https://www.ncbi.nlm.nih.gov/pubmed/22783115
http://dx.doi.org/10.5941/MYCO.2011.39.4.272
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