Cargando…

Development of a 10,000 Locus Genetic Map of the Sunflower Genome Based on Multiple Crosses

Genetic linkage maps have the potential to facilitate the genetic dissection of complex traits and comparative analyses of genome structure, as well as molecular breeding efforts in species of agronomic importance. Until recently, the majority of such maps was based on relatively low-throughput mark...

Descripción completa

Detalles Bibliográficos
Autores principales: Bowers, John E., Bachlava, Eleni, Brunick, Robert L., Rieseberg, Loren H., Knapp, Steven J., Burke, John M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385978/
https://www.ncbi.nlm.nih.gov/pubmed/22870395
http://dx.doi.org/10.1534/g3.112.002659
_version_ 1782236927934595072
author Bowers, John E.
Bachlava, Eleni
Brunick, Robert L.
Rieseberg, Loren H.
Knapp, Steven J.
Burke, John M.
author_facet Bowers, John E.
Bachlava, Eleni
Brunick, Robert L.
Rieseberg, Loren H.
Knapp, Steven J.
Burke, John M.
author_sort Bowers, John E.
collection PubMed
description Genetic linkage maps have the potential to facilitate the genetic dissection of complex traits and comparative analyses of genome structure, as well as molecular breeding efforts in species of agronomic importance. Until recently, the majority of such maps was based on relatively low-throughput marker technologies, which limited marker density across the genome. The availability of high-throughput genotyping technologies has, however, made possible the efficient development of high-density genetic maps. Here, we describe the analysis and integration of genotypic data from four sunflower (Helianthus annuus L.) mapping populations to produce a consensus linkage map of the sunflower genome. Although the individual maps (which contained 3500–5500 loci each) were highly colinear, we observed localized variation in recombination rates in several genomic regions. We also observed several gaps up to 26 cM in length that completely lacked mappable markers in individual crosses, presumably due to regions of identity by descent in the mapping parents. Because these regions differed by cross, the consensus map of 10,080 loci contained no such gaps, clearly illustrating the value of simultaneously analyzing multiple mapping populations.
format Online
Article
Text
id pubmed-3385978
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Genetics Society of America
record_format MEDLINE/PubMed
spelling pubmed-33859782012-08-07 Development of a 10,000 Locus Genetic Map of the Sunflower Genome Based on Multiple Crosses Bowers, John E. Bachlava, Eleni Brunick, Robert L. Rieseberg, Loren H. Knapp, Steven J. Burke, John M. G3 (Bethesda) Investigations Genetic linkage maps have the potential to facilitate the genetic dissection of complex traits and comparative analyses of genome structure, as well as molecular breeding efforts in species of agronomic importance. Until recently, the majority of such maps was based on relatively low-throughput marker technologies, which limited marker density across the genome. The availability of high-throughput genotyping technologies has, however, made possible the efficient development of high-density genetic maps. Here, we describe the analysis and integration of genotypic data from four sunflower (Helianthus annuus L.) mapping populations to produce a consensus linkage map of the sunflower genome. Although the individual maps (which contained 3500–5500 loci each) were highly colinear, we observed localized variation in recombination rates in several genomic regions. We also observed several gaps up to 26 cM in length that completely lacked mappable markers in individual crosses, presumably due to regions of identity by descent in the mapping parents. Because these regions differed by cross, the consensus map of 10,080 loci contained no such gaps, clearly illustrating the value of simultaneously analyzing multiple mapping populations. Genetics Society of America 2012-07-01 /pmc/articles/PMC3385978/ /pubmed/22870395 http://dx.doi.org/10.1534/g3.112.002659 Text en Copyright © 2012 Bowers et al.
spellingShingle Investigations
Bowers, John E.
Bachlava, Eleni
Brunick, Robert L.
Rieseberg, Loren H.
Knapp, Steven J.
Burke, John M.
Development of a 10,000 Locus Genetic Map of the Sunflower Genome Based on Multiple Crosses
title Development of a 10,000 Locus Genetic Map of the Sunflower Genome Based on Multiple Crosses
title_full Development of a 10,000 Locus Genetic Map of the Sunflower Genome Based on Multiple Crosses
title_fullStr Development of a 10,000 Locus Genetic Map of the Sunflower Genome Based on Multiple Crosses
title_full_unstemmed Development of a 10,000 Locus Genetic Map of the Sunflower Genome Based on Multiple Crosses
title_short Development of a 10,000 Locus Genetic Map of the Sunflower Genome Based on Multiple Crosses
title_sort development of a 10,000 locus genetic map of the sunflower genome based on multiple crosses
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385978/
https://www.ncbi.nlm.nih.gov/pubmed/22870395
http://dx.doi.org/10.1534/g3.112.002659
work_keys_str_mv AT bowersjohne developmentofa10000locusgeneticmapofthesunflowergenomebasedonmultiplecrosses
AT bachlavaeleni developmentofa10000locusgeneticmapofthesunflowergenomebasedonmultiplecrosses
AT brunickrobertl developmentofa10000locusgeneticmapofthesunflowergenomebasedonmultiplecrosses
AT rieseberglorenh developmentofa10000locusgeneticmapofthesunflowergenomebasedonmultiplecrosses
AT knappstevenj developmentofa10000locusgeneticmapofthesunflowergenomebasedonmultiplecrosses
AT burkejohnm developmentofa10000locusgeneticmapofthesunflowergenomebasedonmultiplecrosses