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ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5

Small guanosine triphosphatase (GTPase) ADP-ribosylation factors (Arfs) regulate membrane traffic and actin reorganization under the strict control of GTPase-activating proteins (GAPs). ARAP1 (Arf GAP with Rho GAP domain, ankyrin repeat, and PH domain 1) is an Arf GAP molecule with multiple PH domai...

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Detalles Bibliográficos
Autores principales: Hasegawa, Junya, Tsujita, Kazuya, Takenawa, Tadaomi, Itoh, Toshiki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386212/
https://www.ncbi.nlm.nih.gov/pubmed/22573888
http://dx.doi.org/10.1091/mbc.E12-01-0017
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author Hasegawa, Junya
Tsujita, Kazuya
Takenawa, Tadaomi
Itoh, Toshiki
author_facet Hasegawa, Junya
Tsujita, Kazuya
Takenawa, Tadaomi
Itoh, Toshiki
author_sort Hasegawa, Junya
collection PubMed
description Small guanosine triphosphatase (GTPase) ADP-ribosylation factors (Arfs) regulate membrane traffic and actin reorganization under the strict control of GTPase-activating proteins (GAPs). ARAP1 (Arf GAP with Rho GAP domain, ankyrin repeat, and PH domain 1) is an Arf GAP molecule with multiple PH domains that recognize phosphatidylinositol 3,4,5-trisphosphate. We found that growth factor stimulation induced localization of ARAP1 to an area of the plasma membrane inside the ring structure of circular dorsal ruffles (CDRs). Moreover, expression of ARAP1 increased the size of the CDR filamentous-actin ring in an Arf GAP activity–dependent manner, whereas smaller CDRs were formed by ARAP1 knockdown. In addition, expression of a dominant-negative mutant of Arf1 and Arf5, the substrates of ARAP1, expanded the size of CDRs, suggesting that the two Arf isoforms regulate ring structure downstream of ARAP1. Therefore our results reveal a novel molecular mechanism of CDR ring size control through the ARAP1–Arf1/5 pathway.
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spelling pubmed-33862122012-09-16 ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5 Hasegawa, Junya Tsujita, Kazuya Takenawa, Tadaomi Itoh, Toshiki Mol Biol Cell Articles Small guanosine triphosphatase (GTPase) ADP-ribosylation factors (Arfs) regulate membrane traffic and actin reorganization under the strict control of GTPase-activating proteins (GAPs). ARAP1 (Arf GAP with Rho GAP domain, ankyrin repeat, and PH domain 1) is an Arf GAP molecule with multiple PH domains that recognize phosphatidylinositol 3,4,5-trisphosphate. We found that growth factor stimulation induced localization of ARAP1 to an area of the plasma membrane inside the ring structure of circular dorsal ruffles (CDRs). Moreover, expression of ARAP1 increased the size of the CDR filamentous-actin ring in an Arf GAP activity–dependent manner, whereas smaller CDRs were formed by ARAP1 knockdown. In addition, expression of a dominant-negative mutant of Arf1 and Arf5, the substrates of ARAP1, expanded the size of CDRs, suggesting that the two Arf isoforms regulate ring structure downstream of ARAP1. Therefore our results reveal a novel molecular mechanism of CDR ring size control through the ARAP1–Arf1/5 pathway. The American Society for Cell Biology 2012-07-01 /pmc/articles/PMC3386212/ /pubmed/22573888 http://dx.doi.org/10.1091/mbc.E12-01-0017 Text en © 2012 Hasegawa et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Hasegawa, Junya
Tsujita, Kazuya
Takenawa, Tadaomi
Itoh, Toshiki
ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5
title ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5
title_full ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5
title_fullStr ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5
title_full_unstemmed ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5
title_short ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5
title_sort arap1 regulates the ring size of circular dorsal ruffles through arf1 and arf5
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386212/
https://www.ncbi.nlm.nih.gov/pubmed/22573888
http://dx.doi.org/10.1091/mbc.E12-01-0017
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