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Tension-dependent nucleosome remodeling at the pericentromere in yeast
Nucleosome positioning is important for the structural integrity of chromosomes. During metaphase the mitotic spindle exerts physical force on pericentromeric chromatin. The cell must adjust the pericentromeric chromatin to accommodate the changing tension resulting from microtubule dynamics to main...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386219/ https://www.ncbi.nlm.nih.gov/pubmed/22593210 http://dx.doi.org/10.1091/mbc.E11-07-0651 |
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author | Verdaasdonk, Jolien S. Gardner, Ryan Stephens, Andrew D. Yeh, Elaine Bloom, Kerry |
author_facet | Verdaasdonk, Jolien S. Gardner, Ryan Stephens, Andrew D. Yeh, Elaine Bloom, Kerry |
author_sort | Verdaasdonk, Jolien S. |
collection | PubMed |
description | Nucleosome positioning is important for the structural integrity of chromosomes. During metaphase the mitotic spindle exerts physical force on pericentromeric chromatin. The cell must adjust the pericentromeric chromatin to accommodate the changing tension resulting from microtubule dynamics to maintain a stable metaphase spindle. Here we examine the effects of spindle-based tension on nucleosome dynamics by measuring the histone turnover of the chromosome arm and the pericentromere during metaphase in the budding yeast Saccharomyces cerevisiae. We find that both histones H2B and H4 exhibit greater turnover in the pericentromere during metaphase. Loss of spindle-based tension by treatment with the microtubule-depolymerizing drug nocodazole or compromising kinetochore function results in reduced histone turnover in the pericentromere. Pericentromeric histone dynamics are influenced by the chromatin-remodeling activities of STH1/NPS1 and ISW2. Sth1p is the ATPase component of the Remodels the Structure of Chromatin (RSC) complex, and Isw2p is an ATP-dependent DNA translocase member of the Imitation Switch (ISWI) subfamily of chromatin-remodeling factors. The balance between displacement and insertion of pericentromeric histones provides a mechanism to accommodate spindle-based tension while maintaining proper chromatin packaging during mitosis. |
format | Online Article Text |
id | pubmed-3386219 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-33862192012-09-16 Tension-dependent nucleosome remodeling at the pericentromere in yeast Verdaasdonk, Jolien S. Gardner, Ryan Stephens, Andrew D. Yeh, Elaine Bloom, Kerry Mol Biol Cell Articles Nucleosome positioning is important for the structural integrity of chromosomes. During metaphase the mitotic spindle exerts physical force on pericentromeric chromatin. The cell must adjust the pericentromeric chromatin to accommodate the changing tension resulting from microtubule dynamics to maintain a stable metaphase spindle. Here we examine the effects of spindle-based tension on nucleosome dynamics by measuring the histone turnover of the chromosome arm and the pericentromere during metaphase in the budding yeast Saccharomyces cerevisiae. We find that both histones H2B and H4 exhibit greater turnover in the pericentromere during metaphase. Loss of spindle-based tension by treatment with the microtubule-depolymerizing drug nocodazole or compromising kinetochore function results in reduced histone turnover in the pericentromere. Pericentromeric histone dynamics are influenced by the chromatin-remodeling activities of STH1/NPS1 and ISW2. Sth1p is the ATPase component of the Remodels the Structure of Chromatin (RSC) complex, and Isw2p is an ATP-dependent DNA translocase member of the Imitation Switch (ISWI) subfamily of chromatin-remodeling factors. The balance between displacement and insertion of pericentromeric histones provides a mechanism to accommodate spindle-based tension while maintaining proper chromatin packaging during mitosis. The American Society for Cell Biology 2012-07-01 /pmc/articles/PMC3386219/ /pubmed/22593210 http://dx.doi.org/10.1091/mbc.E11-07-0651 Text en © 2012 Verdaasdonk et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Verdaasdonk, Jolien S. Gardner, Ryan Stephens, Andrew D. Yeh, Elaine Bloom, Kerry Tension-dependent nucleosome remodeling at the pericentromere in yeast |
title | Tension-dependent nucleosome remodeling at the pericentromere in yeast |
title_full | Tension-dependent nucleosome remodeling at the pericentromere in yeast |
title_fullStr | Tension-dependent nucleosome remodeling at the pericentromere in yeast |
title_full_unstemmed | Tension-dependent nucleosome remodeling at the pericentromere in yeast |
title_short | Tension-dependent nucleosome remodeling at the pericentromere in yeast |
title_sort | tension-dependent nucleosome remodeling at the pericentromere in yeast |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386219/ https://www.ncbi.nlm.nih.gov/pubmed/22593210 http://dx.doi.org/10.1091/mbc.E11-07-0651 |
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