A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols...

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Detalles Bibliográficos
Autores principales: Guo, Yixing, Yang, Xiaofeng, Hakuna, Lovemore, Barve, Aabha, Escobedo, Jorge O., Lowry, Mark, Strongin, Robert M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386722/
https://www.ncbi.nlm.nih.gov/pubmed/22778623
http://dx.doi.org/10.3390/s120505940
Descripción
Sumario:A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.

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