A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols...

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Detalles Bibliográficos
Autores principales: Guo, Yixing, Yang, Xiaofeng, Hakuna, Lovemore, Barve, Aabha, Escobedo, Jorge O., Lowry, Mark, Strongin, Robert M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386722/
https://www.ncbi.nlm.nih.gov/pubmed/22778623
http://dx.doi.org/10.3390/s120505940
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author Guo, Yixing
Yang, Xiaofeng
Hakuna, Lovemore
Barve, Aabha
Escobedo, Jorge O.
Lowry, Mark
Strongin, Robert M.
author_facet Guo, Yixing
Yang, Xiaofeng
Hakuna, Lovemore
Barve, Aabha
Escobedo, Jorge O.
Lowry, Mark
Strongin, Robert M.
author_sort Guo, Yixing
collection PubMed
description A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
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spelling pubmed-33867222012-07-09 A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma Guo, Yixing Yang, Xiaofeng Hakuna, Lovemore Barve, Aabha Escobedo, Jorge O. Lowry, Mark Strongin, Robert M. Sensors (Basel) Communication A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%. Molecular Diversity Preservation International (MDPI) 2012-05-08 /pmc/articles/PMC3386722/ /pubmed/22778623 http://dx.doi.org/10.3390/s120505940 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Guo, Yixing
Yang, Xiaofeng
Hakuna, Lovemore
Barve, Aabha
Escobedo, Jorge O.
Lowry, Mark
Strongin, Robert M.
A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_full A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_fullStr A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_full_unstemmed A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_short A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_sort fast response highly selective probe for the detection of glutathione in human blood plasma
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386722/
https://www.ncbi.nlm.nih.gov/pubmed/22778623
http://dx.doi.org/10.3390/s120505940
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