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luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?

AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function;...

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Autores principales: Wang, Qian, He, Zhiyan, Hu, Yuejian, Jiang, Yuntao, Ma, Rui, Tang, Zisheng, Liang, Jingping, Liu, Zheng, Huang, Zhengwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386735/
https://www.ncbi.nlm.nih.gov/pubmed/22778636
http://dx.doi.org/10.3390/s120506176
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author Wang, Qian
He, Zhiyan
Hu, Yuejian
Jiang, Yuntao
Ma, Rui
Tang, Zisheng
Liang, Jingping
Liu, Zheng
Huang, Zhengwei
author_facet Wang, Qian
He, Zhiyan
Hu, Yuejian
Jiang, Yuntao
Ma, Rui
Tang, Zisheng
Liang, Jingping
Liu, Zheng
Huang, Zhengwei
author_sort Wang, Qian
collection PubMed
description AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by luxS-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system—not the metabolic aspect—is responsible for such a regulatory function. In this study, we constructed plasmids infused with sahH and induced protein expression in the luxS-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for luxS mutant regulation.
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spelling pubmed-33867352012-07-09 luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? Wang, Qian He, Zhiyan Hu, Yuejian Jiang, Yuntao Ma, Rui Tang, Zisheng Liang, Jingping Liu, Zheng Huang, Zhengwei Sensors (Basel) Article AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by luxS-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system—not the metabolic aspect—is responsible for such a regulatory function. In this study, we constructed plasmids infused with sahH and induced protein expression in the luxS-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for luxS mutant regulation. Molecular Diversity Preservation International (MDPI) 2012-05-10 /pmc/articles/PMC3386735/ /pubmed/22778636 http://dx.doi.org/10.3390/s120506176 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Qian
He, Zhiyan
Hu, Yuejian
Jiang, Yuntao
Ma, Rui
Tang, Zisheng
Liang, Jingping
Liu, Zheng
Huang, Zhengwei
luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?
title luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?
title_full luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?
title_fullStr luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?
title_full_unstemmed luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?
title_short luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?
title_sort luxs mutant regulation: quorum sensing impairment or methylation disorder?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386735/
https://www.ncbi.nlm.nih.gov/pubmed/22778636
http://dx.doi.org/10.3390/s120506176
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