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luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?
AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function;...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386735/ https://www.ncbi.nlm.nih.gov/pubmed/22778636 http://dx.doi.org/10.3390/s120506176 |
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author | Wang, Qian He, Zhiyan Hu, Yuejian Jiang, Yuntao Ma, Rui Tang, Zisheng Liang, Jingping Liu, Zheng Huang, Zhengwei |
author_facet | Wang, Qian He, Zhiyan Hu, Yuejian Jiang, Yuntao Ma, Rui Tang, Zisheng Liang, Jingping Liu, Zheng Huang, Zhengwei |
author_sort | Wang, Qian |
collection | PubMed |
description | AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by luxS-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system—not the metabolic aspect—is responsible for such a regulatory function. In this study, we constructed plasmids infused with sahH and induced protein expression in the luxS-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for luxS mutant regulation. |
format | Online Article Text |
id | pubmed-3386735 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-33867352012-07-09 luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? Wang, Qian He, Zhiyan Hu, Yuejian Jiang, Yuntao Ma, Rui Tang, Zisheng Liang, Jingping Liu, Zheng Huang, Zhengwei Sensors (Basel) Article AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by luxS-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system—not the metabolic aspect—is responsible for such a regulatory function. In this study, we constructed plasmids infused with sahH and induced protein expression in the luxS-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for luxS mutant regulation. Molecular Diversity Preservation International (MDPI) 2012-05-10 /pmc/articles/PMC3386735/ /pubmed/22778636 http://dx.doi.org/10.3390/s120506176 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Wang, Qian He, Zhiyan Hu, Yuejian Jiang, Yuntao Ma, Rui Tang, Zisheng Liang, Jingping Liu, Zheng Huang, Zhengwei luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
title | luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
title_full | luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
title_fullStr | luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
title_full_unstemmed | luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
title_short | luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
title_sort | luxs mutant regulation: quorum sensing impairment or methylation disorder? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386735/ https://www.ncbi.nlm.nih.gov/pubmed/22778636 http://dx.doi.org/10.3390/s120506176 |
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