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Transcriptome Analysis of the Octopus vulgaris Central Nervous System
BACKGROUND: Cephalopoda are a class of Mollusca species found in all the world's oceans. They are an important model organism in neurobiology. Unfortunately, the lack of neuronal molecular sequences, such as ESTs, transcriptomic or genomic information, has limited the development of molecular n...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387010/ https://www.ncbi.nlm.nih.gov/pubmed/22768275 http://dx.doi.org/10.1371/journal.pone.0040320 |
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author | Zhang, Xiang Mao, Yong Huang, Zixia Qu, Meng Chen, Jun Ding, Shaoxiong Hong, Jingni Sun, Tiantian |
author_facet | Zhang, Xiang Mao, Yong Huang, Zixia Qu, Meng Chen, Jun Ding, Shaoxiong Hong, Jingni Sun, Tiantian |
author_sort | Zhang, Xiang |
collection | PubMed |
description | BACKGROUND: Cephalopoda are a class of Mollusca species found in all the world's oceans. They are an important model organism in neurobiology. Unfortunately, the lack of neuronal molecular sequences, such as ESTs, transcriptomic or genomic information, has limited the development of molecular neurobiology research in this unique model organism. RESULTS: With high-throughput Illumina Solexa sequencing technology, we have generated 59,859 high quality sequences from 12,918,391 paired-end reads. Using BLASTx/BLASTn, 12,227 contigs have blast hits in the Swissprot, NR protein database and NT nucleotide database with E-value cutoff 1e(−5). The comparison between the Octopus vulgaris central nervous system (CNS) library and the Aplysia californica/Lymnaea stagnalis CNS ESTs library yielded 5.93%/13.45% of O. vulgaris sequences with significant matches (1e(−5)) using BLASTn/tBLASTx. Meanwhile the hit percentage of the recently published Schistocerca gregaria, Tilapia or Hirudo medicinalis CNS library to the O. vulgaris CNS library is 21.03%–46.19%. We constructed the Phylogenetic tree using two genes related to CNS function, Synaptotagmin-7 and Synaptophysin. Lastly, we demonstrated that O. vulgaris may have a vertebrate-like Blood-Brain Barrier based on bioinformatic analysis. CONCLUSION: This study provides a mass of molecular information that will contribute to further molecular biology research on O. vulgaris. In our presentation of the first CNS transcriptome analysis of O. vulgaris, we hope to accelerate the study of functional molecular neurobiology and comparative evolutionary biology. |
format | Online Article Text |
id | pubmed-3387010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33870102012-07-05 Transcriptome Analysis of the Octopus vulgaris Central Nervous System Zhang, Xiang Mao, Yong Huang, Zixia Qu, Meng Chen, Jun Ding, Shaoxiong Hong, Jingni Sun, Tiantian PLoS One Research Article BACKGROUND: Cephalopoda are a class of Mollusca species found in all the world's oceans. They are an important model organism in neurobiology. Unfortunately, the lack of neuronal molecular sequences, such as ESTs, transcriptomic or genomic information, has limited the development of molecular neurobiology research in this unique model organism. RESULTS: With high-throughput Illumina Solexa sequencing technology, we have generated 59,859 high quality sequences from 12,918,391 paired-end reads. Using BLASTx/BLASTn, 12,227 contigs have blast hits in the Swissprot, NR protein database and NT nucleotide database with E-value cutoff 1e(−5). The comparison between the Octopus vulgaris central nervous system (CNS) library and the Aplysia californica/Lymnaea stagnalis CNS ESTs library yielded 5.93%/13.45% of O. vulgaris sequences with significant matches (1e(−5)) using BLASTn/tBLASTx. Meanwhile the hit percentage of the recently published Schistocerca gregaria, Tilapia or Hirudo medicinalis CNS library to the O. vulgaris CNS library is 21.03%–46.19%. We constructed the Phylogenetic tree using two genes related to CNS function, Synaptotagmin-7 and Synaptophysin. Lastly, we demonstrated that O. vulgaris may have a vertebrate-like Blood-Brain Barrier based on bioinformatic analysis. CONCLUSION: This study provides a mass of molecular information that will contribute to further molecular biology research on O. vulgaris. In our presentation of the first CNS transcriptome analysis of O. vulgaris, we hope to accelerate the study of functional molecular neurobiology and comparative evolutionary biology. Public Library of Science 2012-06-29 /pmc/articles/PMC3387010/ /pubmed/22768275 http://dx.doi.org/10.1371/journal.pone.0040320 Text en Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhang, Xiang Mao, Yong Huang, Zixia Qu, Meng Chen, Jun Ding, Shaoxiong Hong, Jingni Sun, Tiantian Transcriptome Analysis of the Octopus vulgaris Central Nervous System |
title | Transcriptome Analysis of the Octopus vulgaris Central Nervous System |
title_full | Transcriptome Analysis of the Octopus vulgaris Central Nervous System |
title_fullStr | Transcriptome Analysis of the Octopus vulgaris Central Nervous System |
title_full_unstemmed | Transcriptome Analysis of the Octopus vulgaris Central Nervous System |
title_short | Transcriptome Analysis of the Octopus vulgaris Central Nervous System |
title_sort | transcriptome analysis of the octopus vulgaris central nervous system |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387010/ https://www.ncbi.nlm.nih.gov/pubmed/22768275 http://dx.doi.org/10.1371/journal.pone.0040320 |
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