A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate

Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describe a technique that couples the simult...

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Detalles Bibliográficos
Autores principales: McMullen, David C, Kean, William S, Verma, Ajay, Cole, Jeffrey T, Watson, William D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388579/
https://www.ncbi.nlm.nih.gov/pubmed/22472432
http://dx.doi.org/10.1186/1480-9222-14-4
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author McMullen, David C
Kean, William S
Verma, Ajay
Cole, Jeffrey T
Watson, William D
author_facet McMullen, David C
Kean, William S
Verma, Ajay
Cole, Jeffrey T
Watson, William D
author_sort McMullen, David C
collection PubMed
description Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describe a technique that couples the simultaneous quantification of radio-labeled calcium accumulation in endoplasmic reticulum (ER) microsomes with the release of inorganic phosphate (Pi) by the hydrolytic activity of sarco-endoplasmic reticulum calcium ATPase (SERCA) all in the convenience of a 96-well format.
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spelling pubmed-33885792012-07-03 A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate McMullen, David C Kean, William S Verma, Ajay Cole, Jeffrey T Watson, William D Biol Proced Online Methodology Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describe a technique that couples the simultaneous quantification of radio-labeled calcium accumulation in endoplasmic reticulum (ER) microsomes with the release of inorganic phosphate (Pi) by the hydrolytic activity of sarco-endoplasmic reticulum calcium ATPase (SERCA) all in the convenience of a 96-well format. BioMed Central 2012-04-02 /pmc/articles/PMC3388579/ /pubmed/22472432 http://dx.doi.org/10.1186/1480-9222-14-4 Text en Copyright ©2012 McMullen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
McMullen, David C
Kean, William S
Verma, Ajay
Cole, Jeffrey T
Watson, William D
A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate
title A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate
title_full A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate
title_fullStr A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate
title_full_unstemmed A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate
title_short A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate
title_sort microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and serca release of inorganic phosphate
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388579/
https://www.ncbi.nlm.nih.gov/pubmed/22472432
http://dx.doi.org/10.1186/1480-9222-14-4
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