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Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells

BACKGROUND: Adipose stem cells have a strong potential for use in cell-based therapy, but the current nucleofection technique, which relies on unknown buffers, prevents their use. RESULTS: We developed an optimal nucleofection formulation for human adipose stem cells by using a three-step method tha...

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Detalles Bibliográficos
Autores principales: Flanagan, Michael, Gimble, Jeffrey M, Yu, Gang, Xia, Xueqing, Bunnell, Bruce A, Li, Shulin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388581/
https://www.ncbi.nlm.nih.gov/pubmed/22512891
http://dx.doi.org/10.1186/1480-9222-14-7