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Hematological and Immunological Changes Due to Short-term Oral Administration of Acephate
To evaluate immunotoxicological effects of environmental chemical, subacute toxicity of repeated (28 day) oral administration of acephate (Ace) in BALB/c mice was assessed. Thirty two (sixteen male and sixteen female) mice were divided into four different groups with each group containing eight (fou...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388761/ https://www.ncbi.nlm.nih.gov/pubmed/22778515 http://dx.doi.org/10.4103/0971-6580.97217 |
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author | Sankhala, Laxmi N. Tripathi, Syamantak M. Bhavsar, S. K. Thaker, Aswin M. Sharma, Pramod |
author_facet | Sankhala, Laxmi N. Tripathi, Syamantak M. Bhavsar, S. K. Thaker, Aswin M. Sharma, Pramod |
author_sort | Sankhala, Laxmi N. |
collection | PubMed |
description | To evaluate immunotoxicological effects of environmental chemical, subacute toxicity of repeated (28 day) oral administration of acephate (Ace) in BALB/c mice was assessed. Thirty two (sixteen male and sixteen female) mice were divided into four different groups with each group containing eight (four male and four female) mice. Mice of Group C1 were administered normal saline only and served as control. Group T1 was given 1/40(th) of apparent LD(50) (ALD(50)) (8.78 mg/kg), and group T2 was put on 1/30(th) of ALD(50) [11.7 mg/kg], while group T3 received 1/20(th) of ALD(50) [17.55 mg/kg] of Ace suspended in normal saline. The blood samples were collected from mice after 28 days of oral administration and analyzed for hematological, biochemical, and immunological parameters. The study showed that hematological parameters (monocytes and granulocytes) remained unaffected except total leukocyte count and lymphocyte which were decreased highly significantly [P≤0.01] in mice of group T3 on the 28(th) day of experiment. Serum total protein (TP) and serum globulin decreased significantly in mice of treatment groups dose dependently; however, no significant change was seen in serum albumin. Progressive increase in live body weight of mice decreased significantly in extremely toxic group only while spleen:body weight ratio decreased significantly in dose-dependent manner. Furthermore, Ace produced suppressed humoral immune response and the delayed-type hypersensitivity response to Sheep red blood cells (SRBCs) was altered nonsignificantly. The results of this study describe the suppression of immune responses following exposure to Ace at low concentrations in experimental mice. |
format | Online Article Text |
id | pubmed-3388761 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-33887612012-07-09 Hematological and Immunological Changes Due to Short-term Oral Administration of Acephate Sankhala, Laxmi N. Tripathi, Syamantak M. Bhavsar, S. K. Thaker, Aswin M. Sharma, Pramod Toxicol Int Original Article To evaluate immunotoxicological effects of environmental chemical, subacute toxicity of repeated (28 day) oral administration of acephate (Ace) in BALB/c mice was assessed. Thirty two (sixteen male and sixteen female) mice were divided into four different groups with each group containing eight (four male and four female) mice. Mice of Group C1 were administered normal saline only and served as control. Group T1 was given 1/40(th) of apparent LD(50) (ALD(50)) (8.78 mg/kg), and group T2 was put on 1/30(th) of ALD(50) [11.7 mg/kg], while group T3 received 1/20(th) of ALD(50) [17.55 mg/kg] of Ace suspended in normal saline. The blood samples were collected from mice after 28 days of oral administration and analyzed for hematological, biochemical, and immunological parameters. The study showed that hematological parameters (monocytes and granulocytes) remained unaffected except total leukocyte count and lymphocyte which were decreased highly significantly [P≤0.01] in mice of group T3 on the 28(th) day of experiment. Serum total protein (TP) and serum globulin decreased significantly in mice of treatment groups dose dependently; however, no significant change was seen in serum albumin. Progressive increase in live body weight of mice decreased significantly in extremely toxic group only while spleen:body weight ratio decreased significantly in dose-dependent manner. Furthermore, Ace produced suppressed humoral immune response and the delayed-type hypersensitivity response to Sheep red blood cells (SRBCs) was altered nonsignificantly. The results of this study describe the suppression of immune responses following exposure to Ace at low concentrations in experimental mice. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3388761/ /pubmed/22778515 http://dx.doi.org/10.4103/0971-6580.97217 Text en Copyright: © Toxicology International http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Sankhala, Laxmi N. Tripathi, Syamantak M. Bhavsar, S. K. Thaker, Aswin M. Sharma, Pramod Hematological and Immunological Changes Due to Short-term Oral Administration of Acephate |
title | Hematological and Immunological Changes Due to Short-term Oral Administration of Acephate |
title_full | Hematological and Immunological Changes Due to Short-term Oral Administration of Acephate |
title_fullStr | Hematological and Immunological Changes Due to Short-term Oral Administration of Acephate |
title_full_unstemmed | Hematological and Immunological Changes Due to Short-term Oral Administration of Acephate |
title_short | Hematological and Immunological Changes Due to Short-term Oral Administration of Acephate |
title_sort | hematological and immunological changes due to short-term oral administration of acephate |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388761/ https://www.ncbi.nlm.nih.gov/pubmed/22778515 http://dx.doi.org/10.4103/0971-6580.97217 |
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