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In vivo localization at the cellular level of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves

Accurate localization of phytoalexins is a key for better understanding their role. This work aims to localize stilbenes, the main phytoalexins of grapevine. The cellular localization of stilbene fluorescence induced by Plasmopara viticola, the agent of downy mildew, was determined in grapevine leav...

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Autores principales: Bellow, Sébastien, Latouche, Gwendal, Brown, Spencer C., Poutaraud, Anne, Cerovic, Zoran G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388817/
https://www.ncbi.nlm.nih.gov/pubmed/22412183
http://dx.doi.org/10.1093/jxb/ers060
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author Bellow, Sébastien
Latouche, Gwendal
Brown, Spencer C.
Poutaraud, Anne
Cerovic, Zoran G.
author_facet Bellow, Sébastien
Latouche, Gwendal
Brown, Spencer C.
Poutaraud, Anne
Cerovic, Zoran G.
author_sort Bellow, Sébastien
collection PubMed
description Accurate localization of phytoalexins is a key for better understanding their role. This work aims to localize stilbenes, the main phytoalexins of grapevine. The cellular localization of stilbene fluorescence induced by Plasmopara viticola, the agent of downy mildew, was determined in grapevine leaves of very susceptible, susceptible, and partially resistant genotypes during infection. Laser scanning confocal microscopy and microspectrofluorimetry were used to acquire UV-excited autofluorescence three-dimensional images and spectra of grapevine leaves 5–6 days after inoculation. This noninvasive technique of investigation in vivo was completed with in vitro spectrofluorimetric studies on pure stilbenes as their fluorescence is largely affected by the physicochemical environment in various leaf compartments. Viscosity was the major physicochemical factor influencing stilbene fluorescence intensity, modifying fluorescence yield by more than two orders of magnitude. Striking differences in the localization of stilbene fluorescence induced by P. viticola were observed between the different genotypes. All inoculated genotypes displayed stilbene fluorescence in cell walls of guard cells and periclinal cell walls of epidermal cells. Higher fluorescence intensity was observed in guard-cell walls than in any other compartment due to increased local viscosity. In addition stilbene fluorescence was found in epidermal cell vacuoles of the susceptible genotype and in the infected spongy parenchyma of the partially resistant genotype. The very susceptible genotype was devoid of fluorescence both in the epidermal vacuoles and the mesophyll. This strongly suggests that the resistance of grapevine leaves to P. viticola is correlated with the pattern of localization of induced stilbenes in host tissues.
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spelling pubmed-33888172012-07-03 In vivo localization at the cellular level of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves Bellow, Sébastien Latouche, Gwendal Brown, Spencer C. Poutaraud, Anne Cerovic, Zoran G. J Exp Bot Research Paper Accurate localization of phytoalexins is a key for better understanding their role. This work aims to localize stilbenes, the main phytoalexins of grapevine. The cellular localization of stilbene fluorescence induced by Plasmopara viticola, the agent of downy mildew, was determined in grapevine leaves of very susceptible, susceptible, and partially resistant genotypes during infection. Laser scanning confocal microscopy and microspectrofluorimetry were used to acquire UV-excited autofluorescence three-dimensional images and spectra of grapevine leaves 5–6 days after inoculation. This noninvasive technique of investigation in vivo was completed with in vitro spectrofluorimetric studies on pure stilbenes as their fluorescence is largely affected by the physicochemical environment in various leaf compartments. Viscosity was the major physicochemical factor influencing stilbene fluorescence intensity, modifying fluorescence yield by more than two orders of magnitude. Striking differences in the localization of stilbene fluorescence induced by P. viticola were observed between the different genotypes. All inoculated genotypes displayed stilbene fluorescence in cell walls of guard cells and periclinal cell walls of epidermal cells. Higher fluorescence intensity was observed in guard-cell walls than in any other compartment due to increased local viscosity. In addition stilbene fluorescence was found in epidermal cell vacuoles of the susceptible genotype and in the infected spongy parenchyma of the partially resistant genotype. The very susceptible genotype was devoid of fluorescence both in the epidermal vacuoles and the mesophyll. This strongly suggests that the resistance of grapevine leaves to P. viticola is correlated with the pattern of localization of induced stilbenes in host tissues. Oxford University Press 2012-06-13 2012-03-12 /pmc/articles/PMC3388817/ /pubmed/22412183 http://dx.doi.org/10.1093/jxb/ers060 Text en © 2012 The Author(s). http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Paper
Bellow, Sébastien
Latouche, Gwendal
Brown, Spencer C.
Poutaraud, Anne
Cerovic, Zoran G.
In vivo localization at the cellular level of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves
title In vivo localization at the cellular level of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves
title_full In vivo localization at the cellular level of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves
title_fullStr In vivo localization at the cellular level of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves
title_full_unstemmed In vivo localization at the cellular level of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves
title_short In vivo localization at the cellular level of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves
title_sort in vivo localization at the cellular level of stilbene fluorescence induced by plasmopara viticola in grapevine leaves
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388817/
https://www.ncbi.nlm.nih.gov/pubmed/22412183
http://dx.doi.org/10.1093/jxb/ers060
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