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Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
Na-FAR-1 is an unusual α-helix-rich fatty acid- and retinol-binding protein from Necator americanus, a blood-feeding intestinal parasitic nematode of humans. It belongs to the FAR protein family, which is unique to nematodes; no structural information is available to date for FAR proteins from paras...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388935/ https://www.ncbi.nlm.nih.gov/pubmed/22750878 http://dx.doi.org/10.1107/S1744309112023597 |
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author | Gabrielsen, Mads Rey-Burusco, M. Florencia Griffiths, Kate Roe, Andrew J. Cooper, Alan Smith, Brian O. Kennedy, Malcolm W. Corsico, Betina |
author_facet | Gabrielsen, Mads Rey-Burusco, M. Florencia Griffiths, Kate Roe, Andrew J. Cooper, Alan Smith, Brian O. Kennedy, Malcolm W. Corsico, Betina |
author_sort | Gabrielsen, Mads |
collection | PubMed |
description | Na-FAR-1 is an unusual α-helix-rich fatty acid- and retinol-binding protein from Necator americanus, a blood-feeding intestinal parasitic nematode of humans. It belongs to the FAR protein family, which is unique to nematodes; no structural information is available to date for FAR proteins from parasites. Crystals were obtained with two different morphologies that corresponded to different space groups. Crystal form 1 exhibited space group P432 (unit-cell parameters a = b = c = 120.80 Å, α = β = γ = 90°) and diffracted to 2.5 Å resolution, whereas crystal form 2 exhibited space group F23 (unit-cell parameters a = b = c = 240.38 Å, α = β = γ = 90°) and diffracted to 3.2 Å resolution. Crystal form 2 showed signs of significant twinning. |
format | Online Article Text |
id | pubmed-3388935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-33889352012-08-06 Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus Gabrielsen, Mads Rey-Burusco, M. Florencia Griffiths, Kate Roe, Andrew J. Cooper, Alan Smith, Brian O. Kennedy, Malcolm W. Corsico, Betina Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications Na-FAR-1 is an unusual α-helix-rich fatty acid- and retinol-binding protein from Necator americanus, a blood-feeding intestinal parasitic nematode of humans. It belongs to the FAR protein family, which is unique to nematodes; no structural information is available to date for FAR proteins from parasites. Crystals were obtained with two different morphologies that corresponded to different space groups. Crystal form 1 exhibited space group P432 (unit-cell parameters a = b = c = 120.80 Å, α = β = γ = 90°) and diffracted to 2.5 Å resolution, whereas crystal form 2 exhibited space group F23 (unit-cell parameters a = b = c = 240.38 Å, α = β = γ = 90°) and diffracted to 3.2 Å resolution. Crystal form 2 showed signs of significant twinning. International Union of Crystallography 2012-06-28 /pmc/articles/PMC3388935/ /pubmed/22750878 http://dx.doi.org/10.1107/S1744309112023597 Text en © Gabrielsen et al. 2012 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Crystallization Communications Gabrielsen, Mads Rey-Burusco, M. Florencia Griffiths, Kate Roe, Andrew J. Cooper, Alan Smith, Brian O. Kennedy, Malcolm W. Corsico, Betina Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus |
title | Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
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title_full | Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
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title_fullStr | Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
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title_full_unstemmed | Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
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title_short | Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
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title_sort | two crystal forms of a helix-rich fatty acid- and retinol-binding protein, na-far-1, from the parasitic nematode necator americanus |
topic | Crystallization Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388935/ https://www.ncbi.nlm.nih.gov/pubmed/22750878 http://dx.doi.org/10.1107/S1744309112023597 |
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