Cargando…

Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus

Na-FAR-1 is an unusual α-helix-rich fatty acid- and retinol-binding protein from Necator americanus, a blood-feeding intestinal parasitic nematode of humans. It belongs to the FAR protein family, which is unique to nematodes; no structural information is available to date for FAR proteins from paras...

Descripción completa

Detalles Bibliográficos
Autores principales: Gabrielsen, Mads, Rey-Burusco, M. Florencia, Griffiths, Kate, Roe, Andrew J., Cooper, Alan, Smith, Brian O., Kennedy, Malcolm W., Corsico, Betina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388935/
https://www.ncbi.nlm.nih.gov/pubmed/22750878
http://dx.doi.org/10.1107/S1744309112023597
_version_ 1782237258746691584
author Gabrielsen, Mads
Rey-Burusco, M. Florencia
Griffiths, Kate
Roe, Andrew J.
Cooper, Alan
Smith, Brian O.
Kennedy, Malcolm W.
Corsico, Betina
author_facet Gabrielsen, Mads
Rey-Burusco, M. Florencia
Griffiths, Kate
Roe, Andrew J.
Cooper, Alan
Smith, Brian O.
Kennedy, Malcolm W.
Corsico, Betina
author_sort Gabrielsen, Mads
collection PubMed
description Na-FAR-1 is an unusual α-helix-rich fatty acid- and retinol-binding protein from Necator americanus, a blood-feeding intestinal parasitic nematode of humans. It belongs to the FAR protein family, which is unique to nematodes; no structural information is available to date for FAR proteins from parasites. Crystals were obtained with two different morphologies that corresponded to different space groups. Crystal form 1 exhibited space group P432 (unit-cell parameters a = b = c = 120.80 Å, α = β = γ = 90°) and diffracted to 2.5 Å resolution, whereas crystal form 2 exhibited space group F23 (unit-cell parameters a = b = c = 240.38 Å, α = β = γ = 90°) and diffracted to 3.2 Å resolution. Crystal form 2 showed signs of significant twinning.
format Online
Article
Text
id pubmed-3388935
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher International Union of Crystallography
record_format MEDLINE/PubMed
spelling pubmed-33889352012-08-06 Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus Gabrielsen, Mads Rey-Burusco, M. Florencia Griffiths, Kate Roe, Andrew J. Cooper, Alan Smith, Brian O. Kennedy, Malcolm W. Corsico, Betina Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications Na-FAR-1 is an unusual α-helix-rich fatty acid- and retinol-binding protein from Necator americanus, a blood-feeding intestinal parasitic nematode of humans. It belongs to the FAR protein family, which is unique to nematodes; no structural information is available to date for FAR proteins from parasites. Crystals were obtained with two different morphologies that corresponded to different space groups. Crystal form 1 exhibited space group P432 (unit-cell parameters a = b = c = 120.80 Å, α = β = γ = 90°) and diffracted to 2.5 Å resolution, whereas crystal form 2 exhibited space group F23 (unit-cell parameters a = b = c = 240.38 Å, α = β = γ = 90°) and diffracted to 3.2 Å resolution. Crystal form 2 showed signs of significant twinning. International Union of Crystallography 2012-06-28 /pmc/articles/PMC3388935/ /pubmed/22750878 http://dx.doi.org/10.1107/S1744309112023597 Text en © Gabrielsen et al. 2012 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Crystallization Communications
Gabrielsen, Mads
Rey-Burusco, M. Florencia
Griffiths, Kate
Roe, Andrew J.
Cooper, Alan
Smith, Brian O.
Kennedy, Malcolm W.
Corsico, Betina
Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
title Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
title_full Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
title_fullStr Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
title_full_unstemmed Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
title_short Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
title_sort two crystal forms of a helix-rich fatty acid- and retinol-binding protein, na-far-1, from the parasitic nematode necator americanus
topic Crystallization Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388935/
https://www.ncbi.nlm.nih.gov/pubmed/22750878
http://dx.doi.org/10.1107/S1744309112023597
work_keys_str_mv AT gabrielsenmads twocrystalformsofahelixrichfattyacidandretinolbindingproteinnafar1fromtheparasiticnematodenecatoramericanus
AT reyburuscomflorencia twocrystalformsofahelixrichfattyacidandretinolbindingproteinnafar1fromtheparasiticnematodenecatoramericanus
AT griffithskate twocrystalformsofahelixrichfattyacidandretinolbindingproteinnafar1fromtheparasiticnematodenecatoramericanus
AT roeandrewj twocrystalformsofahelixrichfattyacidandretinolbindingproteinnafar1fromtheparasiticnematodenecatoramericanus
AT cooperalan twocrystalformsofahelixrichfattyacidandretinolbindingproteinnafar1fromtheparasiticnematodenecatoramericanus
AT smithbriano twocrystalformsofahelixrichfattyacidandretinolbindingproteinnafar1fromtheparasiticnematodenecatoramericanus
AT kennedymalcolmw twocrystalformsofahelixrichfattyacidandretinolbindingproteinnafar1fromtheparasiticnematodenecatoramericanus
AT corsicobetina twocrystalformsofahelixrichfattyacidandretinolbindingproteinnafar1fromtheparasiticnematodenecatoramericanus