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Differential premature termination of transcription as a proposed mechanism for the regulation of coronavirus gene expression.

We propose that the different subgenomic mRNA levels of coronaviruses are controlled through differential premature termination of transcription, and are modulated by the relative strength of transcriptional initiation/blockage events. We present the complete set of sequences covering the leader enc...

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Detalles Bibliográficos
Autores principales: Konings, D A, Bredenbeek, P J, Noten, J F, Hogeweg, P, Spaan, W J
Formato: Texto
Lenguaje:English
Publicado: 1988
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC338943/
https://www.ncbi.nlm.nih.gov/pubmed/2905039
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author Konings, D A
Bredenbeek, P J
Noten, J F
Hogeweg, P
Spaan, W J
author_facet Konings, D A
Bredenbeek, P J
Noten, J F
Hogeweg, P
Spaan, W J
author_sort Konings, D A
collection PubMed
description We propose that the different subgenomic mRNA levels of coronaviruses are controlled through differential premature termination of transcription, and are modulated by the relative strength of transcriptional initiation/blockage events. We present the complete set of sequences covering the leader encoding and intergenic regions of the MHV-A59 strain. A computer-assisted analysis of the two now complete sets of these sequences of strain IBV-M42 and MHV-A59 shows that, in contrast to the previous theory, differences amongst stabilities of intermolecular base-pairings between the leader and the intergenic regions are not sufficient to determine the mRNA gradients in both MHV and IBV infected cells. Neither can the accessibility of the interacting regions on the leader and the negative stranded genome, as revealed by secondary structure analysis, explain the mRNA levels. The nested gene organisation itself, on the other hand, could be responsible for observed mRNA levels gradually increasing with gene order. Relatively slow new initiation events at intergenic regions are proposed to block elongation of passing transcripts which, via temporary pausing, can cause premature termination of transcription. This effects longer transcripts more than shorter ones.
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spelling pubmed-3389432004-02-25 Differential premature termination of transcription as a proposed mechanism for the regulation of coronavirus gene expression. Konings, D A Bredenbeek, P J Noten, J F Hogeweg, P Spaan, W J Nucleic Acids Res We propose that the different subgenomic mRNA levels of coronaviruses are controlled through differential premature termination of transcription, and are modulated by the relative strength of transcriptional initiation/blockage events. We present the complete set of sequences covering the leader encoding and intergenic regions of the MHV-A59 strain. A computer-assisted analysis of the two now complete sets of these sequences of strain IBV-M42 and MHV-A59 shows that, in contrast to the previous theory, differences amongst stabilities of intermolecular base-pairings between the leader and the intergenic regions are not sufficient to determine the mRNA gradients in both MHV and IBV infected cells. Neither can the accessibility of the interacting regions on the leader and the negative stranded genome, as revealed by secondary structure analysis, explain the mRNA levels. The nested gene organisation itself, on the other hand, could be responsible for observed mRNA levels gradually increasing with gene order. Relatively slow new initiation events at intergenic regions are proposed to block elongation of passing transcripts which, via temporary pausing, can cause premature termination of transcription. This effects longer transcripts more than shorter ones. 1988-11-25 /pmc/articles/PMC338943/ /pubmed/2905039 Text en
spellingShingle Konings, D A
Bredenbeek, P J
Noten, J F
Hogeweg, P
Spaan, W J
Differential premature termination of transcription as a proposed mechanism for the regulation of coronavirus gene expression.
title Differential premature termination of transcription as a proposed mechanism for the regulation of coronavirus gene expression.
title_full Differential premature termination of transcription as a proposed mechanism for the regulation of coronavirus gene expression.
title_fullStr Differential premature termination of transcription as a proposed mechanism for the regulation of coronavirus gene expression.
title_full_unstemmed Differential premature termination of transcription as a proposed mechanism for the regulation of coronavirus gene expression.
title_short Differential premature termination of transcription as a proposed mechanism for the regulation of coronavirus gene expression.
title_sort differential premature termination of transcription as a proposed mechanism for the regulation of coronavirus gene expression.
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC338943/
https://www.ncbi.nlm.nih.gov/pubmed/2905039
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