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Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR

Background. Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. Methods. 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sa...

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Autores principales: Mahmoudi Rad, Mahnaz, Zafarghandi, Ameneh Sh, Amel Zabihi, Maryam, Tavallaee, Mahkam, Mirdamadi, Yasaman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3389677/
https://www.ncbi.nlm.nih.gov/pubmed/22791958
http://dx.doi.org/10.1155/2012/872169
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author Mahmoudi Rad, Mahnaz
Zafarghandi, Ameneh Sh
Amel Zabihi, Maryam
Tavallaee, Mahkam
Mirdamadi, Yasaman
author_facet Mahmoudi Rad, Mahnaz
Zafarghandi, Ameneh Sh
Amel Zabihi, Maryam
Tavallaee, Mahkam
Mirdamadi, Yasaman
author_sort Mahmoudi Rad, Mahnaz
collection PubMed
description Background. Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. Methods. 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sabouraud Dextrose Agar. The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fragment within the ITS2 region were amplified. The multiplex PCR products were separated by electrophoresis in 2% agarose gel, visualized by staining with ethidium bromide, and photographed. Descriptive statistics, Chi-square test, and Spearman correlation were used to summarize the findings. Results. C. albicans and C. glabrata were the most common species isolated from the specimens. A mix of C. glabrata and C. albicans was the most common mixed infection isolated from the samples. The analysis revealed a significant positive association between older age and infection with C. glabrata isolates (Spearman's rho = 0.89, P = 0.015). Conclusion. Multiplex PCR is a fast, yet reliable method to identify Candida species. C. albicans and then C. glabrata are the two most common causes of vulvovaginal candidiasis. The number of mixed fungal infections is higher among Iranian population compared to international reports.
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spelling pubmed-33896772012-07-12 Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR Mahmoudi Rad, Mahnaz Zafarghandi, Ameneh Sh Amel Zabihi, Maryam Tavallaee, Mahkam Mirdamadi, Yasaman Infect Dis Obstet Gynecol Research Article Background. Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. Methods. 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sabouraud Dextrose Agar. The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fragment within the ITS2 region were amplified. The multiplex PCR products were separated by electrophoresis in 2% agarose gel, visualized by staining with ethidium bromide, and photographed. Descriptive statistics, Chi-square test, and Spearman correlation were used to summarize the findings. Results. C. albicans and C. glabrata were the most common species isolated from the specimens. A mix of C. glabrata and C. albicans was the most common mixed infection isolated from the samples. The analysis revealed a significant positive association between older age and infection with C. glabrata isolates (Spearman's rho = 0.89, P = 0.015). Conclusion. Multiplex PCR is a fast, yet reliable method to identify Candida species. C. albicans and then C. glabrata are the two most common causes of vulvovaginal candidiasis. The number of mixed fungal infections is higher among Iranian population compared to international reports. Hindawi Publishing Corporation 2012 2012-06-26 /pmc/articles/PMC3389677/ /pubmed/22791958 http://dx.doi.org/10.1155/2012/872169 Text en Copyright © 2012 Mahnaz Mahmoudi Rad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mahmoudi Rad, Mahnaz
Zafarghandi, Ameneh Sh
Amel Zabihi, Maryam
Tavallaee, Mahkam
Mirdamadi, Yasaman
Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
title Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
title_full Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
title_fullStr Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
title_full_unstemmed Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
title_short Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
title_sort identification of candida species associated with vulvovaginal candidiasis by multiplex pcr
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3389677/
https://www.ncbi.nlm.nih.gov/pubmed/22791958
http://dx.doi.org/10.1155/2012/872169
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