Coalition of DNA polymorphisms of ApoB and ApoAI genes is related with coronary artery disease in Kazaks

OBJECTIVE: To explore the relationship between polymorphisms of XbaI and MspI loci of apolipoprotein B (ApoB) gene and -75 bp, +83 bp loci of apolipoprotein AI (ApoAI) gene and coronary heart disease (CHD) in Kazaks of Xinjiang Uyghur Autonomous Region, China. METHODS: These loci were analyzed by PCR-restriction fragment length polymorphism (PCR-PFLP). Two hundred and five patients with CHD and two hundred and thirty six controls were involved. RESULTS: There were significant distinctions among low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and the ApoAI/ApoB ratio between the two groups, but no significant distinction among the polymorphism frequencies of the four sites between the two groups. The polymorphism coalition frequency of X(−−)/Ms(++)/M(1)(+−)/M(2)(++) (named Coalition 11) was significantly higher in CHD compared to the control group (14.6% vs. 7.2%, P < 0.05). The level of total cholesterol (TC) in Coalition 11 was significantly higher and the level of the ApoAI/ApoB ratio in Coalition 11 was significantly lower than Coalition 1∼10 in CHD patients. The level of the ApoAI/ApoB ratio of Coalition 11 was significantly lower than the Coalition 1∼10 in control group. The levels of ApoAI/ApoB ratio of Coalition 3 were significantly higher compared to Coalition 11 in the two groups, respectively. The level of LDL-C of Coalition 3 was significantly lower than in the Coalition 11 in control group. The level of TC of Coalition 5 was significantly higher than Coalition 3 in the CHD group. The level of the ApoAI/ApoB ratio of Coalition 5 was significantly lower than in Coalition 3 or Coalition 1∼10 of the two groups, respectively. The level of LDL-C of Coalition 5 was significantly higher than in Coalition 3 in control group. The ratio of ApoAI/ApoB was negatively related to TC, LDL-C and was positively related to HDL-C, both in CHD and control groups. CONCLUSION: Coalition 11 of the 4 loci polymorphisms of the ApoB and ApoAI genes was correlated with CHD in Kazaks, and perhaps the ratio of ApoAI/ApoB was the most diagnostic parameter related with CHD among all lipid parameters. CHD may also be associated with Coalition 5, and, perhaps, Coalition 3 may have been confirmed as a protection factor against CHD, if more samples were enrolled.