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Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells

The usage of stem cells is a promising strategy for the repair of damaged tissue in the injured brain. Recently, amniotic fluid (AF) cells have received a lot of attention as an alternative source of stem cells for cell-based therapies. However, the success of this approach relies significantly on p...

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Autores principales: Jezierski, Anna, Rennie, Kerry, Tremblay, Roger, Zurakowski, Bogdan, Gruslin, Andreé, Sikorska, Marianna, Bani-Yaghoub, Mahmud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3390140/
https://www.ncbi.nlm.nih.gov/pubmed/22792116
http://dx.doi.org/10.1155/2012/607161
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author Jezierski, Anna
Rennie, Kerry
Tremblay, Roger
Zurakowski, Bogdan
Gruslin, Andreé
Sikorska, Marianna
Bani-Yaghoub, Mahmud
author_facet Jezierski, Anna
Rennie, Kerry
Tremblay, Roger
Zurakowski, Bogdan
Gruslin, Andreé
Sikorska, Marianna
Bani-Yaghoub, Mahmud
author_sort Jezierski, Anna
collection PubMed
description The usage of stem cells is a promising strategy for the repair of damaged tissue in the injured brain. Recently, amniotic fluid (AF) cells have received a lot of attention as an alternative source of stem cells for cell-based therapies. However, the success of this approach relies significantly on proper interactions between graft and host tissue. In particular, the reestablishment of functional brain networks requires formation of gap junctions, as a key step to provide sufficient intercellular communication. In this study, we show that AF cells express high levels of CX43 (GJA1) and are able to establish functional gap junctions with cortical cultures. Furthermore, we report an induction of Cx43 expression in astrocytes following injury to the mouse motor cortex and demonstrate for the first time CX43 expression at the interface between implanted AF cells and host brain cells. These findings suggest that CX43-mediated intercellular communication between AF cells and cortical astrocytes may contribute to the reconstruction of damaged tissue by mediating modulatory, homeostatic, and protective factors in the injured brain and hence warrants further investigation.
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spelling pubmed-33901402012-07-12 Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells Jezierski, Anna Rennie, Kerry Tremblay, Roger Zurakowski, Bogdan Gruslin, Andreé Sikorska, Marianna Bani-Yaghoub, Mahmud Stem Cells Int Research Article The usage of stem cells is a promising strategy for the repair of damaged tissue in the injured brain. Recently, amniotic fluid (AF) cells have received a lot of attention as an alternative source of stem cells for cell-based therapies. However, the success of this approach relies significantly on proper interactions between graft and host tissue. In particular, the reestablishment of functional brain networks requires formation of gap junctions, as a key step to provide sufficient intercellular communication. In this study, we show that AF cells express high levels of CX43 (GJA1) and are able to establish functional gap junctions with cortical cultures. Furthermore, we report an induction of Cx43 expression in astrocytes following injury to the mouse motor cortex and demonstrate for the first time CX43 expression at the interface between implanted AF cells and host brain cells. These findings suggest that CX43-mediated intercellular communication between AF cells and cortical astrocytes may contribute to the reconstruction of damaged tissue by mediating modulatory, homeostatic, and protective factors in the injured brain and hence warrants further investigation. Hindawi Publishing Corporation 2012 2012-06-26 /pmc/articles/PMC3390140/ /pubmed/22792116 http://dx.doi.org/10.1155/2012/607161 Text en Copyright © 2012 Anna Jezierski et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jezierski, Anna
Rennie, Kerry
Tremblay, Roger
Zurakowski, Bogdan
Gruslin, Andreé
Sikorska, Marianna
Bani-Yaghoub, Mahmud
Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells
title Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells
title_full Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells
title_fullStr Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells
title_full_unstemmed Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells
title_short Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells
title_sort human amniotic fluid cells form functional gap junctions with cortical cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3390140/
https://www.ncbi.nlm.nih.gov/pubmed/22792116
http://dx.doi.org/10.1155/2012/607161
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