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A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos
Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia. We estimated the diagnostic accuracy of two anti-JEV immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) (Panbio and XCyton JEVCheX) compared with a reference standard (AFRIMS JEV MAC ELISA) in a prospective s...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The American Society of Tropical Medicine and Hygiene
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391045/ https://www.ncbi.nlm.nih.gov/pubmed/22764310 http://dx.doi.org/10.4269/ajtmh.2012.11-0729 |
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author | Moore, Catrin E. Blacksell, Stuart D. Taojaikong, Thaksinaporn Jarman, Richard G. Gibbons, Robert V. Lee, Sue J. Chansamouth, Vilada Thongpaseuth, Soulignasack Mayxay, Mayfong Newton, Paul N. |
author_facet | Moore, Catrin E. Blacksell, Stuart D. Taojaikong, Thaksinaporn Jarman, Richard G. Gibbons, Robert V. Lee, Sue J. Chansamouth, Vilada Thongpaseuth, Soulignasack Mayxay, Mayfong Newton, Paul N. |
author_sort | Moore, Catrin E. |
collection | PubMed |
description | Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia. We estimated the diagnostic accuracy of two anti-JEV immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) (Panbio and XCyton JEVCheX) compared with a reference standard (AFRIMS JEV MAC ELISA) in a prospective study of the causes of central nervous system infections in Laos. Cerebrospinal fluid (CSF; 515 patients) and serum samples (182 patients) from those admitted to Mahosot Hospital, Vientiane, were tested. The CSF from 14.5% of acute encephalitis syndrome (AES) patients and 10.1% from those with AES and meningitis were positive for anti-JEV IgM in the reference ELISA. The sensitivities for CSF were 65.4% (95% confidence interval [CI] = 51–78) (Xcyton), 69.2% (95% CI = 55–81) (Panbio), however 96.2% (95% CI = 87–100) with Panbio Ravi criteria. Specificities were 89–100%. For admission sera from AES patients, sensitivities and specificities of the Panbio ELISA were 85.7% (95% CI = 42–100%) and 92.9% (95% CI = 83–98%), respectively. |
format | Online Article Text |
id | pubmed-3391045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The American Society of Tropical Medicine and Hygiene |
record_format | MEDLINE/PubMed |
spelling | pubmed-33910452012-07-12 A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos Moore, Catrin E. Blacksell, Stuart D. Taojaikong, Thaksinaporn Jarman, Richard G. Gibbons, Robert V. Lee, Sue J. Chansamouth, Vilada Thongpaseuth, Soulignasack Mayxay, Mayfong Newton, Paul N. Am J Trop Med Hyg Articles Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia. We estimated the diagnostic accuracy of two anti-JEV immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) (Panbio and XCyton JEVCheX) compared with a reference standard (AFRIMS JEV MAC ELISA) in a prospective study of the causes of central nervous system infections in Laos. Cerebrospinal fluid (CSF; 515 patients) and serum samples (182 patients) from those admitted to Mahosot Hospital, Vientiane, were tested. The CSF from 14.5% of acute encephalitis syndrome (AES) patients and 10.1% from those with AES and meningitis were positive for anti-JEV IgM in the reference ELISA. The sensitivities for CSF were 65.4% (95% confidence interval [CI] = 51–78) (Xcyton), 69.2% (95% CI = 55–81) (Panbio), however 96.2% (95% CI = 87–100) with Panbio Ravi criteria. Specificities were 89–100%. For admission sera from AES patients, sensitivities and specificities of the Panbio ELISA were 85.7% (95% CI = 42–100%) and 92.9% (95% CI = 83–98%), respectively. The American Society of Tropical Medicine and Hygiene 2012-07-01 /pmc/articles/PMC3391045/ /pubmed/22764310 http://dx.doi.org/10.4269/ajtmh.2012.11-0729 Text en ©The American Society of Tropical Medicine and Hygiene http://creativecommons.org/licenses/by/2.5/ This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Moore, Catrin E. Blacksell, Stuart D. Taojaikong, Thaksinaporn Jarman, Richard G. Gibbons, Robert V. Lee, Sue J. Chansamouth, Vilada Thongpaseuth, Soulignasack Mayxay, Mayfong Newton, Paul N. A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos |
title | A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos |
title_full | A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos |
title_fullStr | A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos |
title_full_unstemmed | A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos |
title_short | A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos |
title_sort | prospective assessment of the accuracy of commercial igm elisas in diagnosis of japanese encephalitis virus infections in patients with suspected central nervous system infections in laos |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391045/ https://www.ncbi.nlm.nih.gov/pubmed/22764310 http://dx.doi.org/10.4269/ajtmh.2012.11-0729 |
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