Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lytic...

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Autores principales: Arash, Ghodousi A, Vatani, S, Darban-Sarokhalil, Davood, Omrani, Maryam, Fooladi, AA, Khosaravi, AD, Feizabadi, Mohammad Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391561/
https://www.ncbi.nlm.nih.gov/pubmed/22783461
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author Arash, Ghodousi A
Vatani, S
Darban-Sarokhalil, Davood
Omrani, Maryam
Fooladi, AA
Khosaravi, AD
Feizabadi, Mohammad Mehdi
author_facet Arash, Ghodousi A
Vatani, S
Darban-Sarokhalil, Davood
Omrani, Maryam
Fooladi, AA
Khosaravi, AD
Feizabadi, Mohammad Mehdi
author_sort Arash, Ghodousi A
collection PubMed
description A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.
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spelling pubmed-33915612012-07-10 Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex Arash, Ghodousi A Vatani, S Darban-Sarokhalil, Davood Omrani, Maryam Fooladi, AA Khosaravi, AD Feizabadi, Mohammad Mehdi Iran J Microbiol Original Article A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria. Tehran University of Medical Sciences 2012-03 /pmc/articles/PMC3391561/ /pubmed/22783461 Text en © 2012 Iranian Society of Microbiology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Arash, Ghodousi A
Vatani, S
Darban-Sarokhalil, Davood
Omrani, Maryam
Fooladi, AA
Khosaravi, AD
Feizabadi, Mohammad Mehdi
Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_full Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_fullStr Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_full_unstemmed Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_short Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_sort development of a new dna extraction protocol for pfge typing of mycobacterium tuberculosis complex
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391561/
https://www.ncbi.nlm.nih.gov/pubmed/22783461
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