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Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe
Hydrogen sulfide is an essential gasotransmitter associated with numerous pathologies. We assert that hydrogen sulfide plays an important role in regulating macrophage function in response to subsequent inflammatory stimuli, promoting clearance of leukocyte infiltrate and reducing TNF-α levels in vi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391661/ https://www.ncbi.nlm.nih.gov/pubmed/22787557 http://dx.doi.org/10.1038/srep00499 |
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author | Dufton, Neil Natividad, Jane Verdu, Elena F. Wallace, John L. |
author_facet | Dufton, Neil Natividad, Jane Verdu, Elena F. Wallace, John L. |
author_sort | Dufton, Neil |
collection | PubMed |
description | Hydrogen sulfide is an essential gasotransmitter associated with numerous pathologies. We assert that hydrogen sulfide plays an important role in regulating macrophage function in response to subsequent inflammatory stimuli, promoting clearance of leukocyte infiltrate and reducing TNF-α levels in vivo following zymosan-challenge. We describe two distinct methods of measuring leukocyte hydrogen sulfide synthesis; methylene blue formation following zinc acetate capture and a novel fluorescent sulfidefluor probe. Comparison of these methods, using pharmacological tools, revealed they were complimentary in vitro and in vivo. We demonstrate the application of sulfidefluor probe to spectrofluorimetry, flow cytometry and whole animal imaging, to monitor the regulation of hydrogen sulfide synthesis in vivo during dynamic inflammatory processes. Both methodologies revealed that granulocyte infiltration negatively affects hydrogen sulfide synthesis. Our report offers an insight into the profile of hydrogen sulfide synthesis during inflammation and highlight opportunities raised by the development of novel fluorescent hydrogen sulfide probes. |
format | Online Article Text |
id | pubmed-3391661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-33916612012-07-11 Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe Dufton, Neil Natividad, Jane Verdu, Elena F. Wallace, John L. Sci Rep Article Hydrogen sulfide is an essential gasotransmitter associated with numerous pathologies. We assert that hydrogen sulfide plays an important role in regulating macrophage function in response to subsequent inflammatory stimuli, promoting clearance of leukocyte infiltrate and reducing TNF-α levels in vivo following zymosan-challenge. We describe two distinct methods of measuring leukocyte hydrogen sulfide synthesis; methylene blue formation following zinc acetate capture and a novel fluorescent sulfidefluor probe. Comparison of these methods, using pharmacological tools, revealed they were complimentary in vitro and in vivo. We demonstrate the application of sulfidefluor probe to spectrofluorimetry, flow cytometry and whole animal imaging, to monitor the regulation of hydrogen sulfide synthesis in vivo during dynamic inflammatory processes. Both methodologies revealed that granulocyte infiltration negatively affects hydrogen sulfide synthesis. Our report offers an insight into the profile of hydrogen sulfide synthesis during inflammation and highlight opportunities raised by the development of novel fluorescent hydrogen sulfide probes. Nature Publishing Group 2012-07-09 /pmc/articles/PMC3391661/ /pubmed/22787557 http://dx.doi.org/10.1038/srep00499 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Dufton, Neil Natividad, Jane Verdu, Elena F. Wallace, John L. Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe |
title | Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe |
title_full | Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe |
title_fullStr | Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe |
title_full_unstemmed | Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe |
title_short | Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe |
title_sort | hydrogen sulfide and resolution of acute inflammation: a comparative study utilizing a novel fluorescent probe |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391661/ https://www.ncbi.nlm.nih.gov/pubmed/22787557 http://dx.doi.org/10.1038/srep00499 |
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