RNAi promotes heterochromatic silencing through replication-coupled release of RNA polII

Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification (1) during the DNA replication phase of the cell cycle(2). Here, we show that the alternati...

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Detalles Bibliográficos
Autores principales: Zaratiegui, Mikel, Castel, Stephane, Irvine, Danielle V., Kloc, Anna, Ren, Jie, Li, Fei, de Castro, Elisa, Marín, Laura, Chang, An-Yun, Goto, Derek, Cande, W. Zacheus, Antequera, Francisco, Arcangioli, Benoit, Martienssen, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391703/
https://www.ncbi.nlm.nih.gov/pubmed/22002604
http://dx.doi.org/10.1038/nature10501
Descripción
Sumario:Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification (1) during the DNA replication phase of the cell cycle(2). Here, we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication. Co-transcriptional RNAi releases RNA polymerase II (PolII), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes(3) which spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.

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