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Alteration of drug metabolizing enzymes in sulphite oxidase deficiency
The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinki...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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the Society for Free Radical Research Japan
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391863/ https://www.ncbi.nlm.nih.gov/pubmed/22798713 http://dx.doi.org/10.3164/jcbn.11-79 |
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author | Tutuncu, Begum Kuçukatay, Vural Arslan, Sevki Sahin, Barbaros Semiz, Asli Sen, Alaattin |
author_facet | Tutuncu, Begum Kuçukatay, Vural Arslan, Sevki Sahin, Barbaros Semiz, Asli Sen, Alaattin |
author_sort | Tutuncu, Begum |
collection | PubMed |
description | The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient groups was measured to confirm SOX deficiency. Then, aminopyrine N-demethylase, aniline 4-hydroxylase, aromatase, caffeine N-demethylase, cytochrome b5 reductase, erythromycin N-demethylase, ethoxyresorufin O-deethylase, glutathione S-transferase, N-nitrosodimethylamine N-demethylase and penthoxyresorufin O-deethylase activities were determined to follow changes in the activity of drug metabolizing enzymes in SOX-deficient rats. Our results clearly demonstrated that SOX deficiency significantly elevated A4H, caffeine N-demethylase, erythromycin N-demethylase and N-nitrosodimethylamine N-demethylase activities while decreasing ethoxyresorufin O-deethylase and aromatase activities. These alterations in drug metabolizing enzymes can contribute to the varying susceptibility and response of sulphite-sensitive individuals to different drugs and/or therapeutics used for treatments. |
format | Online Article Text |
id | pubmed-3391863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | the Society for Free Radical Research Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-33918632012-07-13 Alteration of drug metabolizing enzymes in sulphite oxidase deficiency Tutuncu, Begum Kuçukatay, Vural Arslan, Sevki Sahin, Barbaros Semiz, Asli Sen, Alaattin J Clin Biochem Nutr Original Article The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient groups was measured to confirm SOX deficiency. Then, aminopyrine N-demethylase, aniline 4-hydroxylase, aromatase, caffeine N-demethylase, cytochrome b5 reductase, erythromycin N-demethylase, ethoxyresorufin O-deethylase, glutathione S-transferase, N-nitrosodimethylamine N-demethylase and penthoxyresorufin O-deethylase activities were determined to follow changes in the activity of drug metabolizing enzymes in SOX-deficient rats. Our results clearly demonstrated that SOX deficiency significantly elevated A4H, caffeine N-demethylase, erythromycin N-demethylase and N-nitrosodimethylamine N-demethylase activities while decreasing ethoxyresorufin O-deethylase and aromatase activities. These alterations in drug metabolizing enzymes can contribute to the varying susceptibility and response of sulphite-sensitive individuals to different drugs and/or therapeutics used for treatments. the Society for Free Radical Research Japan 2012-07 2012-03-30 /pmc/articles/PMC3391863/ /pubmed/22798713 http://dx.doi.org/10.3164/jcbn.11-79 Text en Copyright © 2012 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Tutuncu, Begum Kuçukatay, Vural Arslan, Sevki Sahin, Barbaros Semiz, Asli Sen, Alaattin Alteration of drug metabolizing enzymes in sulphite oxidase deficiency |
title | Alteration of drug metabolizing enzymes in sulphite oxidase deficiency |
title_full | Alteration of drug metabolizing enzymes in sulphite oxidase deficiency |
title_fullStr | Alteration of drug metabolizing enzymes in sulphite oxidase deficiency |
title_full_unstemmed | Alteration of drug metabolizing enzymes in sulphite oxidase deficiency |
title_short | Alteration of drug metabolizing enzymes in sulphite oxidase deficiency |
title_sort | alteration of drug metabolizing enzymes in sulphite oxidase deficiency |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391863/ https://www.ncbi.nlm.nih.gov/pubmed/22798713 http://dx.doi.org/10.3164/jcbn.11-79 |
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