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Alteration of drug metabolizing enzymes in sulphite oxidase deficiency

The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinki...

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Autores principales: Tutuncu, Begum, Kuçukatay, Vural, Arslan, Sevki, Sahin, Barbaros, Semiz, Asli, Sen, Alaattin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391863/
https://www.ncbi.nlm.nih.gov/pubmed/22798713
http://dx.doi.org/10.3164/jcbn.11-79
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author Tutuncu, Begum
Kuçukatay, Vural
Arslan, Sevki
Sahin, Barbaros
Semiz, Asli
Sen, Alaattin
author_facet Tutuncu, Begum
Kuçukatay, Vural
Arslan, Sevki
Sahin, Barbaros
Semiz, Asli
Sen, Alaattin
author_sort Tutuncu, Begum
collection PubMed
description The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient groups was measured to confirm SOX deficiency. Then, aminopyrine N-demethylase, aniline 4-hydroxylase, aromatase, caffeine N-demethylase, cytochrome b5 reductase, erythromycin N-demethylase, ethoxyresorufin O-deethylase, glutathione S-transferase, N-nitrosodimethylamine N-demethylase and penthoxyresorufin O-deethylase activities were determined to follow changes in the activity of drug metabolizing enzymes in SOX-deficient rats. Our results clearly demonstrated that SOX deficiency significantly elevated A4H, caffeine N-demethylase, erythromycin N-demethylase and N-nitrosodimethylamine N-demethylase activities while decreasing ethoxyresorufin O-deethylase and aromatase activities. These alterations in drug metabolizing enzymes can contribute to the varying susceptibility and response of sulphite-sensitive individuals to different drugs and/or therapeutics used for treatments.
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spelling pubmed-33918632012-07-13 Alteration of drug metabolizing enzymes in sulphite oxidase deficiency Tutuncu, Begum Kuçukatay, Vural Arslan, Sevki Sahin, Barbaros Semiz, Asli Sen, Alaattin J Clin Biochem Nutr Original Article The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient groups was measured to confirm SOX deficiency. Then, aminopyrine N-demethylase, aniline 4-hydroxylase, aromatase, caffeine N-demethylase, cytochrome b5 reductase, erythromycin N-demethylase, ethoxyresorufin O-deethylase, glutathione S-transferase, N-nitrosodimethylamine N-demethylase and penthoxyresorufin O-deethylase activities were determined to follow changes in the activity of drug metabolizing enzymes in SOX-deficient rats. Our results clearly demonstrated that SOX deficiency significantly elevated A4H, caffeine N-demethylase, erythromycin N-demethylase and N-nitrosodimethylamine N-demethylase activities while decreasing ethoxyresorufin O-deethylase and aromatase activities. These alterations in drug metabolizing enzymes can contribute to the varying susceptibility and response of sulphite-sensitive individuals to different drugs and/or therapeutics used for treatments. the Society for Free Radical Research Japan 2012-07 2012-03-30 /pmc/articles/PMC3391863/ /pubmed/22798713 http://dx.doi.org/10.3164/jcbn.11-79 Text en Copyright © 2012 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Tutuncu, Begum
Kuçukatay, Vural
Arslan, Sevki
Sahin, Barbaros
Semiz, Asli
Sen, Alaattin
Alteration of drug metabolizing enzymes in sulphite oxidase deficiency
title Alteration of drug metabolizing enzymes in sulphite oxidase deficiency
title_full Alteration of drug metabolizing enzymes in sulphite oxidase deficiency
title_fullStr Alteration of drug metabolizing enzymes in sulphite oxidase deficiency
title_full_unstemmed Alteration of drug metabolizing enzymes in sulphite oxidase deficiency
title_short Alteration of drug metabolizing enzymes in sulphite oxidase deficiency
title_sort alteration of drug metabolizing enzymes in sulphite oxidase deficiency
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391863/
https://www.ncbi.nlm.nih.gov/pubmed/22798713
http://dx.doi.org/10.3164/jcbn.11-79
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