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Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va

Leishmaniasis is a neglected tropical disease with no effective vaccines. Actin, microtubules and the actin-based molecular motor myosin Va were investigated for their involvement in Leishmania braziliensis macrophage interactions. Results showed a decrease in the association index when macrophages...

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Autores principales: Azevedo, Elisama, Oliveira, Leandro Teixeira, Castro Lima, Ana Karina, Terra, Rodrigo, Dutra, Patrícia Maria Lourenço, Salerno, Verônica P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391898/
https://www.ncbi.nlm.nih.gov/pubmed/22792440
http://dx.doi.org/10.1155/2012/275436
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author Azevedo, Elisama
Oliveira, Leandro Teixeira
Castro Lima, Ana Karina
Terra, Rodrigo
Dutra, Patrícia Maria Lourenço
Salerno, Verônica P.
author_facet Azevedo, Elisama
Oliveira, Leandro Teixeira
Castro Lima, Ana Karina
Terra, Rodrigo
Dutra, Patrícia Maria Lourenço
Salerno, Verônica P.
author_sort Azevedo, Elisama
collection PubMed
description Leishmaniasis is a neglected tropical disease with no effective vaccines. Actin, microtubules and the actin-based molecular motor myosin Va were investigated for their involvement in Leishmania braziliensis macrophage interactions. Results showed a decrease in the association index when macrophages were without F-actin or microtubules regardless of the activation state of the macrophage. In the absence of F-actin, the production of NO in non-activated cells increased, while in activated cells, the production of NO was reduced independent of parasites. The opposite effect of an increased NO production was observed in the absence of microtubules. In activated cells, the loss of cytoskeletal components inhibited the release of IL-10 during parasite interactions. The production of IL-10 also decreased in the absence of actin or microtubules in non-activated macrophages. Only the disruption of actin altered the production of TNF-α in activated macrophages. The expression of myosin Va tail resulted in an acute decrease in the association index between transfected macrophages and L. braziliensis promastigotes. These data reveal the importance of F-actin, microtubules, and myosin-Va suggesting that modulation of the cytoskeleton may be a mechanism used by L. braziliensis to overcome the natural responses of macrophages to establish infections.
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spelling pubmed-33918982012-07-12 Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va Azevedo, Elisama Oliveira, Leandro Teixeira Castro Lima, Ana Karina Terra, Rodrigo Dutra, Patrícia Maria Lourenço Salerno, Verônica P. J Parasitol Res Research Article Leishmaniasis is a neglected tropical disease with no effective vaccines. Actin, microtubules and the actin-based molecular motor myosin Va were investigated for their involvement in Leishmania braziliensis macrophage interactions. Results showed a decrease in the association index when macrophages were without F-actin or microtubules regardless of the activation state of the macrophage. In the absence of F-actin, the production of NO in non-activated cells increased, while in activated cells, the production of NO was reduced independent of parasites. The opposite effect of an increased NO production was observed in the absence of microtubules. In activated cells, the loss of cytoskeletal components inhibited the release of IL-10 during parasite interactions. The production of IL-10 also decreased in the absence of actin or microtubules in non-activated macrophages. Only the disruption of actin altered the production of TNF-α in activated macrophages. The expression of myosin Va tail resulted in an acute decrease in the association index between transfected macrophages and L. braziliensis promastigotes. These data reveal the importance of F-actin, microtubules, and myosin-Va suggesting that modulation of the cytoskeleton may be a mechanism used by L. braziliensis to overcome the natural responses of macrophages to establish infections. Hindawi Publishing Corporation 2012 2012-06-27 /pmc/articles/PMC3391898/ /pubmed/22792440 http://dx.doi.org/10.1155/2012/275436 Text en Copyright © 2012 Elisama Azevedo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Azevedo, Elisama
Oliveira, Leandro Teixeira
Castro Lima, Ana Karina
Terra, Rodrigo
Dutra, Patrícia Maria Lourenço
Salerno, Verônica P.
Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va
title Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va
title_full Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va
title_fullStr Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va
title_full_unstemmed Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va
title_short Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va
title_sort interactions between leishmania braziliensis and macrophages are dependent on the cytoskeleton and myosin va
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391898/
https://www.ncbi.nlm.nih.gov/pubmed/22792440
http://dx.doi.org/10.1155/2012/275436
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