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Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va
Leishmaniasis is a neglected tropical disease with no effective vaccines. Actin, microtubules and the actin-based molecular motor myosin Va were investigated for their involvement in Leishmania braziliensis macrophage interactions. Results showed a decrease in the association index when macrophages...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391898/ https://www.ncbi.nlm.nih.gov/pubmed/22792440 http://dx.doi.org/10.1155/2012/275436 |
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author | Azevedo, Elisama Oliveira, Leandro Teixeira Castro Lima, Ana Karina Terra, Rodrigo Dutra, Patrícia Maria Lourenço Salerno, Verônica P. |
author_facet | Azevedo, Elisama Oliveira, Leandro Teixeira Castro Lima, Ana Karina Terra, Rodrigo Dutra, Patrícia Maria Lourenço Salerno, Verônica P. |
author_sort | Azevedo, Elisama |
collection | PubMed |
description | Leishmaniasis is a neglected tropical disease with no effective vaccines. Actin, microtubules and the actin-based molecular motor myosin Va were investigated for their involvement in Leishmania braziliensis macrophage interactions. Results showed a decrease in the association index when macrophages were without F-actin or microtubules regardless of the activation state of the macrophage. In the absence of F-actin, the production of NO in non-activated cells increased, while in activated cells, the production of NO was reduced independent of parasites. The opposite effect of an increased NO production was observed in the absence of microtubules. In activated cells, the loss of cytoskeletal components inhibited the release of IL-10 during parasite interactions. The production of IL-10 also decreased in the absence of actin or microtubules in non-activated macrophages. Only the disruption of actin altered the production of TNF-α in activated macrophages. The expression of myosin Va tail resulted in an acute decrease in the association index between transfected macrophages and L. braziliensis promastigotes. These data reveal the importance of F-actin, microtubules, and myosin-Va suggesting that modulation of the cytoskeleton may be a mechanism used by L. braziliensis to overcome the natural responses of macrophages to establish infections. |
format | Online Article Text |
id | pubmed-3391898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33918982012-07-12 Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va Azevedo, Elisama Oliveira, Leandro Teixeira Castro Lima, Ana Karina Terra, Rodrigo Dutra, Patrícia Maria Lourenço Salerno, Verônica P. J Parasitol Res Research Article Leishmaniasis is a neglected tropical disease with no effective vaccines. Actin, microtubules and the actin-based molecular motor myosin Va were investigated for their involvement in Leishmania braziliensis macrophage interactions. Results showed a decrease in the association index when macrophages were without F-actin or microtubules regardless of the activation state of the macrophage. In the absence of F-actin, the production of NO in non-activated cells increased, while in activated cells, the production of NO was reduced independent of parasites. The opposite effect of an increased NO production was observed in the absence of microtubules. In activated cells, the loss of cytoskeletal components inhibited the release of IL-10 during parasite interactions. The production of IL-10 also decreased in the absence of actin or microtubules in non-activated macrophages. Only the disruption of actin altered the production of TNF-α in activated macrophages. The expression of myosin Va tail resulted in an acute decrease in the association index between transfected macrophages and L. braziliensis promastigotes. These data reveal the importance of F-actin, microtubules, and myosin-Va suggesting that modulation of the cytoskeleton may be a mechanism used by L. braziliensis to overcome the natural responses of macrophages to establish infections. Hindawi Publishing Corporation 2012 2012-06-27 /pmc/articles/PMC3391898/ /pubmed/22792440 http://dx.doi.org/10.1155/2012/275436 Text en Copyright © 2012 Elisama Azevedo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Azevedo, Elisama Oliveira, Leandro Teixeira Castro Lima, Ana Karina Terra, Rodrigo Dutra, Patrícia Maria Lourenço Salerno, Verônica P. Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
title | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
title_full | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
title_fullStr | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
title_full_unstemmed | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
title_short | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
title_sort | interactions between leishmania braziliensis and macrophages are dependent on the cytoskeleton and myosin va |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391898/ https://www.ncbi.nlm.nih.gov/pubmed/22792440 http://dx.doi.org/10.1155/2012/275436 |
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