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Advanced Corneal Cross-Linking System with Fluorescence Dosimetry
Purpose. This paper describes an advanced system that combines corneal cross-linking with riboflavin with fluorescence dosimetry, the ability to measure riboflavin diffusion within the cornea both before and during UVA treatment. Methods and Results. A corneal cross-linking system utilizing a digita...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391900/ https://www.ncbi.nlm.nih.gov/pubmed/22792444 http://dx.doi.org/10.1155/2012/303459 |
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author | Friedman, Marc D. Pertaub, Radha Usher, David Sherr, Evan Kamaev, Pavel Muller, David |
author_facet | Friedman, Marc D. Pertaub, Radha Usher, David Sherr, Evan Kamaev, Pavel Muller, David |
author_sort | Friedman, Marc D. |
collection | PubMed |
description | Purpose. This paper describes an advanced system that combines corneal cross-linking with riboflavin with fluorescence dosimetry, the ability to measure riboflavin diffusion within the cornea both before and during UVA treatment. Methods and Results. A corneal cross-linking system utilizing a digital micromirror device (DMD) was assembled and used to measure diffusion coefficients of 0.1% riboflavin in 20% dextran in porcine eyes. A value of (3.3 ± 0.2) × 10(−7) cm(2)/s was obtained for the stroma. Diffusion coefficients for the transepithelial formulation of 0.1% riboflavin in 0.44% saline and 0.02% BAK were also measured to be 4.7 ± 0.3 × 10(−8) cm(2)/s for epithelium only and (4.6 ± 0.4) × 10(−7) cm(2)/s for stroma only. Riboflavin consumption during a UVA treatment was also demonstrated. Conclusion. A new advanced corneal cross-linking system with fluorescence dosimetry of riboflavin has been demonstrated. It is hoped that this method may play a significant role in determining the underlying mechanisms of corneal cross-linking and assist with the development of additional riboflavin formulations. Moreover, dosimetry may prove valuable in providing a method to account for the biological differences between individuals, potentially informing cornea-specific UVA treatment doses in real time. |
format | Online Article Text |
id | pubmed-3391900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33919002012-07-12 Advanced Corneal Cross-Linking System with Fluorescence Dosimetry Friedman, Marc D. Pertaub, Radha Usher, David Sherr, Evan Kamaev, Pavel Muller, David J Ophthalmol Research Article Purpose. This paper describes an advanced system that combines corneal cross-linking with riboflavin with fluorescence dosimetry, the ability to measure riboflavin diffusion within the cornea both before and during UVA treatment. Methods and Results. A corneal cross-linking system utilizing a digital micromirror device (DMD) was assembled and used to measure diffusion coefficients of 0.1% riboflavin in 20% dextran in porcine eyes. A value of (3.3 ± 0.2) × 10(−7) cm(2)/s was obtained for the stroma. Diffusion coefficients for the transepithelial formulation of 0.1% riboflavin in 0.44% saline and 0.02% BAK were also measured to be 4.7 ± 0.3 × 10(−8) cm(2)/s for epithelium only and (4.6 ± 0.4) × 10(−7) cm(2)/s for stroma only. Riboflavin consumption during a UVA treatment was also demonstrated. Conclusion. A new advanced corneal cross-linking system with fluorescence dosimetry of riboflavin has been demonstrated. It is hoped that this method may play a significant role in determining the underlying mechanisms of corneal cross-linking and assist with the development of additional riboflavin formulations. Moreover, dosimetry may prove valuable in providing a method to account for the biological differences between individuals, potentially informing cornea-specific UVA treatment doses in real time. Hindawi Publishing Corporation 2012 2012-06-27 /pmc/articles/PMC3391900/ /pubmed/22792444 http://dx.doi.org/10.1155/2012/303459 Text en Copyright © 2012 Marc D. Friedman et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Friedman, Marc D. Pertaub, Radha Usher, David Sherr, Evan Kamaev, Pavel Muller, David Advanced Corneal Cross-Linking System with Fluorescence Dosimetry |
title | Advanced Corneal Cross-Linking System with Fluorescence Dosimetry |
title_full | Advanced Corneal Cross-Linking System with Fluorescence Dosimetry |
title_fullStr | Advanced Corneal Cross-Linking System with Fluorescence Dosimetry |
title_full_unstemmed | Advanced Corneal Cross-Linking System with Fluorescence Dosimetry |
title_short | Advanced Corneal Cross-Linking System with Fluorescence Dosimetry |
title_sort | advanced corneal cross-linking system with fluorescence dosimetry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391900/ https://www.ncbi.nlm.nih.gov/pubmed/22792444 http://dx.doi.org/10.1155/2012/303459 |
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