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Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies

Contemporary studies in forensic entomology exhaustively evaluate gene sequences because these constitute the fastest and most accurate method of species identification. For this purpose single gene segments, cytochrome oxidase subunit I (COI) in particular, are commonly used. However, the limitatio...

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Autores principales: Zaidi, Farrah, Wei, Shu-jun, Shi, Min, Chen, Xue-xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391916/
https://www.ncbi.nlm.nih.gov/pubmed/21864153
http://dx.doi.org/10.1673/031.011.5901
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author Zaidi, Farrah
Wei, Shu-jun
Shi, Min
Chen, Xue-xin
author_facet Zaidi, Farrah
Wei, Shu-jun
Shi, Min
Chen, Xue-xin
author_sort Zaidi, Farrah
collection PubMed
description Contemporary studies in forensic entomology exhaustively evaluate gene sequences because these constitute the fastest and most accurate method of species identification. For this purpose single gene segments, cytochrome oxidase subunit I (COI) in particular, are commonly used. However, the limitation of such sequences in identification, especially of closely related species and populations, demand a multi-gene approach. But this raises the question of which group of genes can best fulfill the identification task? In this context the utility of five gene segments was explored among blowfly species from two distinct geographic regions, China and Pakistan. COI, cytochrome b (CYTB), NADH dehydrogenase 5 (ND5), nuclear internal transcribed spacers (ITS1 and ITS2), were sequenced for eight blowfly species including Chrysomya megacephala F. (Diptera: Calliphoidae), Ch. pinguis Walker, Lucilia sericata Meigen L. porphyrina Walker, L. illustris Meigen Hemipyrellia ligurriens Wiedemann, Aldrichina grahami Aldrich, and the housefly, Musca domestica L. (Muscidae), from Hangzhou, China; while COI, CYTB, and ITS2 were sequenced for four species, i.e. Ch. megacephala, Ch. rufifacies, L. cuprina, and the flesh fly, Sarcophaga albiceps Meigen (Sarcophagidae), from Dera Ismail Khan Pakistan. The results demonstrate a universal utility of these gene segments in the molecular identification of flies of forensic importance.
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spelling pubmed-33919162012-07-09 Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies Zaidi, Farrah Wei, Shu-jun Shi, Min Chen, Xue-xin J Insect Sci Article Contemporary studies in forensic entomology exhaustively evaluate gene sequences because these constitute the fastest and most accurate method of species identification. For this purpose single gene segments, cytochrome oxidase subunit I (COI) in particular, are commonly used. However, the limitation of such sequences in identification, especially of closely related species and populations, demand a multi-gene approach. But this raises the question of which group of genes can best fulfill the identification task? In this context the utility of five gene segments was explored among blowfly species from two distinct geographic regions, China and Pakistan. COI, cytochrome b (CYTB), NADH dehydrogenase 5 (ND5), nuclear internal transcribed spacers (ITS1 and ITS2), were sequenced for eight blowfly species including Chrysomya megacephala F. (Diptera: Calliphoidae), Ch. pinguis Walker, Lucilia sericata Meigen L. porphyrina Walker, L. illustris Meigen Hemipyrellia ligurriens Wiedemann, Aldrichina grahami Aldrich, and the housefly, Musca domestica L. (Muscidae), from Hangzhou, China; while COI, CYTB, and ITS2 were sequenced for four species, i.e. Ch. megacephala, Ch. rufifacies, L. cuprina, and the flesh fly, Sarcophaga albiceps Meigen (Sarcophagidae), from Dera Ismail Khan Pakistan. The results demonstrate a universal utility of these gene segments in the molecular identification of flies of forensic importance. University of Wisconsin Library 2011-05-05 /pmc/articles/PMC3391916/ /pubmed/21864153 http://dx.doi.org/10.1673/031.011.5901 Text en © 2011 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Zaidi, Farrah
Wei, Shu-jun
Shi, Min
Chen, Xue-xin
Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies
title Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies
title_full Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies
title_fullStr Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies
title_full_unstemmed Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies
title_short Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies
title_sort utility of multi-gene loci for forensic species diagnosis of blowflies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391916/
https://www.ncbi.nlm.nih.gov/pubmed/21864153
http://dx.doi.org/10.1673/031.011.5901
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