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Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions

Human leukaemic HL-60 cells are widely used for studying interactions involving adhesion molecules [e.g. P-selectin and PSGL-1 (P-selectin glycoprotein ligand-1)] since their rolling behaviour has been shown to mimic the dynamics of leucocyte rolling in vitro. HL-60 cells are neutrophilic promyelocy...

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Autores principales: Gee, David J., Wright, L. Kate, Zimmermann, Jonathan, Cole, Kayla, Soule, Karen, Ubowski, Michelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392102/
https://www.ncbi.nlm.nih.gov/pubmed/22494057
http://dx.doi.org/10.1042/BSR20110109
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author Gee, David J.
Wright, L. Kate
Zimmermann, Jonathan
Cole, Kayla
Soule, Karen
Ubowski, Michelle
author_facet Gee, David J.
Wright, L. Kate
Zimmermann, Jonathan
Cole, Kayla
Soule, Karen
Ubowski, Michelle
author_sort Gee, David J.
collection PubMed
description Human leukaemic HL-60 cells are widely used for studying interactions involving adhesion molecules [e.g. P-selectin and PSGL-1 (P-selectin glycoprotein ligand-1)] since their rolling behaviour has been shown to mimic the dynamics of leucocyte rolling in vitro. HL-60 cells are neutrophilic promyelocytes that can undergo granulocytic differentiation upon exposure to compounds such as DMSO (dimethylsulfoxide). Using a parallel plate flow chamber functionalized with recombinant P-selectin–Fc chimaera, undifferentiated and DMSO-induced (48, 72 and 96 h) HL-60 cells were assayed for rolling behaviour. We found that depending on P-selectin incubation concentration, undifferentiated cells incurred up to a 6-fold increase in rolling velocity while subjected to an approximately 10-fold increase in biologically relevant shear stress. HL-60 cells exposed to DMSO for up to 72 h incurred up to a 3-fold increase in rolling velocity over the same shear stress range. Significantly, cells exposed for up to 96 h incurred up to a 9-fold decrease in rolling velocity, compared with undifferentiated HL-60 cells. Although cell surface and nuclear morphological changes were evident upon exposure to DMSO, flow cytometric analysis revealed that PSGL-1 expression was unchanged, irrespective of treatment duration. The results suggest that DMSO-treated HL-60 cells may be problematic as a substitute for neutrophils for trafficking studies during advanced stages of the LAC (leucocyte adhesion cascade). We suggest that remodelling of the cell surface during differentiation may affect rolling behaviour and that DMSO-treated HL-60 cells would behave differently from the normal leucocytes during inflammatory response in vivo.
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spelling pubmed-33921022012-07-12 Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions Gee, David J. Wright, L. Kate Zimmermann, Jonathan Cole, Kayla Soule, Karen Ubowski, Michelle Biosci Rep Original Paper Human leukaemic HL-60 cells are widely used for studying interactions involving adhesion molecules [e.g. P-selectin and PSGL-1 (P-selectin glycoprotein ligand-1)] since their rolling behaviour has been shown to mimic the dynamics of leucocyte rolling in vitro. HL-60 cells are neutrophilic promyelocytes that can undergo granulocytic differentiation upon exposure to compounds such as DMSO (dimethylsulfoxide). Using a parallel plate flow chamber functionalized with recombinant P-selectin–Fc chimaera, undifferentiated and DMSO-induced (48, 72 and 96 h) HL-60 cells were assayed for rolling behaviour. We found that depending on P-selectin incubation concentration, undifferentiated cells incurred up to a 6-fold increase in rolling velocity while subjected to an approximately 10-fold increase in biologically relevant shear stress. HL-60 cells exposed to DMSO for up to 72 h incurred up to a 3-fold increase in rolling velocity over the same shear stress range. Significantly, cells exposed for up to 96 h incurred up to a 9-fold decrease in rolling velocity, compared with undifferentiated HL-60 cells. Although cell surface and nuclear morphological changes were evident upon exposure to DMSO, flow cytometric analysis revealed that PSGL-1 expression was unchanged, irrespective of treatment duration. The results suggest that DMSO-treated HL-60 cells may be problematic as a substitute for neutrophils for trafficking studies during advanced stages of the LAC (leucocyte adhesion cascade). We suggest that remodelling of the cell surface during differentiation may affect rolling behaviour and that DMSO-treated HL-60 cells would behave differently from the normal leucocytes during inflammatory response in vivo. Portland Press Ltd. 2012-05-18 2012-08-01 /pmc/articles/PMC3392102/ /pubmed/22494057 http://dx.doi.org/10.1042/BSR20110109 Text en © 2012 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited
spellingShingle Original Paper
Gee, David J.
Wright, L. Kate
Zimmermann, Jonathan
Cole, Kayla
Soule, Karen
Ubowski, Michelle
Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions
title Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions
title_full Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions
title_fullStr Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions
title_full_unstemmed Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions
title_short Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions
title_sort dimethylsulfoxide exposure modulates hl-60 cell rolling interactions
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392102/
https://www.ncbi.nlm.nih.gov/pubmed/22494057
http://dx.doi.org/10.1042/BSR20110109
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