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Single-Molecule Electrical Random Resequencing of DNA and RNA
Two paradigm shifts in DNA sequencing technologies—from bulk to single molecules and from optical to electrical detection—are expected to realize label-free, low-cost DNA sequencing that does not require PCR amplification. It will lead to development of high-throughput third-generation sequencing te...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392642/ https://www.ncbi.nlm.nih.gov/pubmed/22787559 http://dx.doi.org/10.1038/srep00501 |
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author | Ohshiro, Takahito Matsubara, Kazuki Tsutsui, Makusu Furuhashi, Masayuki Taniguchi, Masateru Kawai, Tomoji |
author_facet | Ohshiro, Takahito Matsubara, Kazuki Tsutsui, Makusu Furuhashi, Masayuki Taniguchi, Masateru Kawai, Tomoji |
author_sort | Ohshiro, Takahito |
collection | PubMed |
description | Two paradigm shifts in DNA sequencing technologies—from bulk to single molecules and from optical to electrical detection—are expected to realize label-free, low-cost DNA sequencing that does not require PCR amplification. It will lead to development of high-throughput third-generation sequencing technologies for personalized medicine. Although nanopore devices have been proposed as third-generation DNA-sequencing devices, a significant milestone in these technologies has been attained by demonstrating a novel technique for resequencing DNA using electrical signals. Here we report single-molecule electrical resequencing of DNA and RNA using a hybrid method of identifying single-base molecules via tunneling currents and random sequencing. Our method reads sequences of nine types of DNA oligomers. The complete sequence of 5′-UGAGGUA-3′ from the let-7 microRNA family was also identified by creating a composite of overlapping fragment sequences, which was randomly determined using tunneling current conducted by single-base molecules as they passed between a pair of nanoelectrodes. |
format | Online Article Text |
id | pubmed-3392642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-33926422012-07-11 Single-Molecule Electrical Random Resequencing of DNA and RNA Ohshiro, Takahito Matsubara, Kazuki Tsutsui, Makusu Furuhashi, Masayuki Taniguchi, Masateru Kawai, Tomoji Sci Rep Article Two paradigm shifts in DNA sequencing technologies—from bulk to single molecules and from optical to electrical detection—are expected to realize label-free, low-cost DNA sequencing that does not require PCR amplification. It will lead to development of high-throughput third-generation sequencing technologies for personalized medicine. Although nanopore devices have been proposed as third-generation DNA-sequencing devices, a significant milestone in these technologies has been attained by demonstrating a novel technique for resequencing DNA using electrical signals. Here we report single-molecule electrical resequencing of DNA and RNA using a hybrid method of identifying single-base molecules via tunneling currents and random sequencing. Our method reads sequences of nine types of DNA oligomers. The complete sequence of 5′-UGAGGUA-3′ from the let-7 microRNA family was also identified by creating a composite of overlapping fragment sequences, which was randomly determined using tunneling current conducted by single-base molecules as they passed between a pair of nanoelectrodes. Nature Publishing Group 2012-07-10 /pmc/articles/PMC3392642/ /pubmed/22787559 http://dx.doi.org/10.1038/srep00501 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
spellingShingle | Article Ohshiro, Takahito Matsubara, Kazuki Tsutsui, Makusu Furuhashi, Masayuki Taniguchi, Masateru Kawai, Tomoji Single-Molecule Electrical Random Resequencing of DNA and RNA |
title | Single-Molecule Electrical Random Resequencing of DNA and RNA |
title_full | Single-Molecule Electrical Random Resequencing of DNA and RNA |
title_fullStr | Single-Molecule Electrical Random Resequencing of DNA and RNA |
title_full_unstemmed | Single-Molecule Electrical Random Resequencing of DNA and RNA |
title_short | Single-Molecule Electrical Random Resequencing of DNA and RNA |
title_sort | single-molecule electrical random resequencing of dna and rna |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392642/ https://www.ncbi.nlm.nih.gov/pubmed/22787559 http://dx.doi.org/10.1038/srep00501 |
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