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Single-Molecule Electrical Random Resequencing of DNA and RNA

Two paradigm shifts in DNA sequencing technologies—from bulk to single molecules and from optical to electrical detection—are expected to realize label-free, low-cost DNA sequencing that does not require PCR amplification. It will lead to development of high-throughput third-generation sequencing te...

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Autores principales: Ohshiro, Takahito, Matsubara, Kazuki, Tsutsui, Makusu, Furuhashi, Masayuki, Taniguchi, Masateru, Kawai, Tomoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392642/
https://www.ncbi.nlm.nih.gov/pubmed/22787559
http://dx.doi.org/10.1038/srep00501
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author Ohshiro, Takahito
Matsubara, Kazuki
Tsutsui, Makusu
Furuhashi, Masayuki
Taniguchi, Masateru
Kawai, Tomoji
author_facet Ohshiro, Takahito
Matsubara, Kazuki
Tsutsui, Makusu
Furuhashi, Masayuki
Taniguchi, Masateru
Kawai, Tomoji
author_sort Ohshiro, Takahito
collection PubMed
description Two paradigm shifts in DNA sequencing technologies—from bulk to single molecules and from optical to electrical detection—are expected to realize label-free, low-cost DNA sequencing that does not require PCR amplification. It will lead to development of high-throughput third-generation sequencing technologies for personalized medicine. Although nanopore devices have been proposed as third-generation DNA-sequencing devices, a significant milestone in these technologies has been attained by demonstrating a novel technique for resequencing DNA using electrical signals. Here we report single-molecule electrical resequencing of DNA and RNA using a hybrid method of identifying single-base molecules via tunneling currents and random sequencing. Our method reads sequences of nine types of DNA oligomers. The complete sequence of 5′-UGAGGUA-3′ from the let-7 microRNA family was also identified by creating a composite of overlapping fragment sequences, which was randomly determined using tunneling current conducted by single-base molecules as they passed between a pair of nanoelectrodes.
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spelling pubmed-33926422012-07-11 Single-Molecule Electrical Random Resequencing of DNA and RNA Ohshiro, Takahito Matsubara, Kazuki Tsutsui, Makusu Furuhashi, Masayuki Taniguchi, Masateru Kawai, Tomoji Sci Rep Article Two paradigm shifts in DNA sequencing technologies—from bulk to single molecules and from optical to electrical detection—are expected to realize label-free, low-cost DNA sequencing that does not require PCR amplification. It will lead to development of high-throughput third-generation sequencing technologies for personalized medicine. Although nanopore devices have been proposed as third-generation DNA-sequencing devices, a significant milestone in these technologies has been attained by demonstrating a novel technique for resequencing DNA using electrical signals. Here we report single-molecule electrical resequencing of DNA and RNA using a hybrid method of identifying single-base molecules via tunneling currents and random sequencing. Our method reads sequences of nine types of DNA oligomers. The complete sequence of 5′-UGAGGUA-3′ from the let-7 microRNA family was also identified by creating a composite of overlapping fragment sequences, which was randomly determined using tunneling current conducted by single-base molecules as they passed between a pair of nanoelectrodes. Nature Publishing Group 2012-07-10 /pmc/articles/PMC3392642/ /pubmed/22787559 http://dx.doi.org/10.1038/srep00501 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Ohshiro, Takahito
Matsubara, Kazuki
Tsutsui, Makusu
Furuhashi, Masayuki
Taniguchi, Masateru
Kawai, Tomoji
Single-Molecule Electrical Random Resequencing of DNA and RNA
title Single-Molecule Electrical Random Resequencing of DNA and RNA
title_full Single-Molecule Electrical Random Resequencing of DNA and RNA
title_fullStr Single-Molecule Electrical Random Resequencing of DNA and RNA
title_full_unstemmed Single-Molecule Electrical Random Resequencing of DNA and RNA
title_short Single-Molecule Electrical Random Resequencing of DNA and RNA
title_sort single-molecule electrical random resequencing of dna and rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392642/
https://www.ncbi.nlm.nih.gov/pubmed/22787559
http://dx.doi.org/10.1038/srep00501
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