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Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest

Leukocyte trafficking to acute sites of injury or infection requires spatial and temporal cues that fine tune precise sites of firm adhesion and guide migration to endothelial junctions where they undergo diapedesis to sites of insult. Many detailed studies on the location and gradient of chemokines...

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Autores principales: Dixit, Neha, Simon, Scott I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392659/
https://www.ncbi.nlm.nih.gov/pubmed/22787461
http://dx.doi.org/10.3389/fimmu.2012.00188
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author Dixit, Neha
Simon, Scott I.
author_facet Dixit, Neha
Simon, Scott I.
author_sort Dixit, Neha
collection PubMed
description Leukocyte trafficking to acute sites of injury or infection requires spatial and temporal cues that fine tune precise sites of firm adhesion and guide migration to endothelial junctions where they undergo diapedesis to sites of insult. Many detailed studies on the location and gradient of chemokines such as IL-8 and other CXCR ligands reveal that their recognition shortly after selectin-mediated capture and rolling exerts acute effects on integrin activation and subsequent binding to their ligands on the endothelium, which directs firm adhesion, adhesion strengthening, and downstream migration. In this process, G-protein coupled receptor (GPCR) signaling has been found to play an integral role in activating and mobilizing intracellular stores of calcium, GTPases such as Rap-1 and Rho and cytokeletal proteins such as Talin and F-actin to facilitate cell polarity and directional pseudopod formation. A critical question remaining is how intracellular Ca(2+) flux from CRAC channels such as Orai1 synergizes with cytosolic stores to mediate a rapid flux which is critical to the onset of PMN arrest and polarization. Our review will highlight a specific role for calcium as a signaling messenger in activating focal clusters of integrins bound to the cytoskeleton which allows the cell to attain a migratory phenotype. The precise interplay between chemokines, selectins, and integrins binding under the ubiquitous presence of shear stress from blood flow provides an essential cooperative signaling mechanism for effective leukocyte recruitment.
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spelling pubmed-33926592012-07-11 Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest Dixit, Neha Simon, Scott I. Front Immunol Immunology Leukocyte trafficking to acute sites of injury or infection requires spatial and temporal cues that fine tune precise sites of firm adhesion and guide migration to endothelial junctions where they undergo diapedesis to sites of insult. Many detailed studies on the location and gradient of chemokines such as IL-8 and other CXCR ligands reveal that their recognition shortly after selectin-mediated capture and rolling exerts acute effects on integrin activation and subsequent binding to their ligands on the endothelium, which directs firm adhesion, adhesion strengthening, and downstream migration. In this process, G-protein coupled receptor (GPCR) signaling has been found to play an integral role in activating and mobilizing intracellular stores of calcium, GTPases such as Rap-1 and Rho and cytokeletal proteins such as Talin and F-actin to facilitate cell polarity and directional pseudopod formation. A critical question remaining is how intracellular Ca(2+) flux from CRAC channels such as Orai1 synergizes with cytosolic stores to mediate a rapid flux which is critical to the onset of PMN arrest and polarization. Our review will highlight a specific role for calcium as a signaling messenger in activating focal clusters of integrins bound to the cytoskeleton which allows the cell to attain a migratory phenotype. The precise interplay between chemokines, selectins, and integrins binding under the ubiquitous presence of shear stress from blood flow provides an essential cooperative signaling mechanism for effective leukocyte recruitment. Frontiers Media S.A. 2012-07-10 /pmc/articles/PMC3392659/ /pubmed/22787461 http://dx.doi.org/10.3389/fimmu.2012.00188 Text en Copyright © 2012 Dixit and Simon. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Immunology
Dixit, Neha
Simon, Scott I.
Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest
title Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest
title_full Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest
title_fullStr Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest
title_full_unstemmed Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest
title_short Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest
title_sort chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392659/
https://www.ncbi.nlm.nih.gov/pubmed/22787461
http://dx.doi.org/10.3389/fimmu.2012.00188
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