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A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots
Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Ext...
Autores principales: | Rodrigues, Elisete Pains, Torres, Adalgisa Ribeiro, da Silva Batista, Jesiane Stefânia, Huergo, Luciano, Hungria, Mariangela |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392887/ https://www.ncbi.nlm.nih.gov/pubmed/22802720 http://dx.doi.org/10.1590/S1415-47572012000200016 |
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