The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA

BACKGROUND: Human immunodeficiency virus type 1 (HIV-1) Nef-encoded protein plays key functions at almost all stages of the viral life cycle, but its role in translation is largely unknown. METHODS: To determine the effect of Nef on translation we used an in vitro translation assay. The detection of...

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Autores principales: Abbas, Wasim, Dichamp, Isabelle, Herbein, Georges
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3393617/
https://www.ncbi.nlm.nih.gov/pubmed/22672539
http://dx.doi.org/10.1186/1743-422X-9-103
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author Abbas, Wasim
Dichamp, Isabelle
Herbein, Georges
author_facet Abbas, Wasim
Dichamp, Isabelle
Herbein, Georges
author_sort Abbas, Wasim
collection PubMed
description BACKGROUND: Human immunodeficiency virus type 1 (HIV-1) Nef-encoded protein plays key functions at almost all stages of the viral life cycle, but its role in translation is largely unknown. METHODS: To determine the effect of Nef on translation we used an in vitro translation assay. The detection of Nef/RPS10 complexes and the presence of 18S rRNA and tRNAs in the complexes were performed by coimmunoprecipitation and RT-PCR assay. RESULTS: We observed that the HIV-1 Nef protein specifically impaired translation in vitro. We observed the interaction of Nef with RPS10 by coimmunoprecipitation assay. In addition 18S rRNA and tRNAs were present in the Nef/RPS10 complexes. CONCLUSIONS: Our results are consistent with a model in which the Nef protein by binding to two components of the 40S small ribosomal subunit, RPS10 and 18S rRNA, and to a lesser extent to tRNAs, could lead to decreased protein synthesis.
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spelling pubmed-33936172012-07-11 The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA Abbas, Wasim Dichamp, Isabelle Herbein, Georges Virol J Research BACKGROUND: Human immunodeficiency virus type 1 (HIV-1) Nef-encoded protein plays key functions at almost all stages of the viral life cycle, but its role in translation is largely unknown. METHODS: To determine the effect of Nef on translation we used an in vitro translation assay. The detection of Nef/RPS10 complexes and the presence of 18S rRNA and tRNAs in the complexes were performed by coimmunoprecipitation and RT-PCR assay. RESULTS: We observed that the HIV-1 Nef protein specifically impaired translation in vitro. We observed the interaction of Nef with RPS10 by coimmunoprecipitation assay. In addition 18S rRNA and tRNAs were present in the Nef/RPS10 complexes. CONCLUSIONS: Our results are consistent with a model in which the Nef protein by binding to two components of the 40S small ribosomal subunit, RPS10 and 18S rRNA, and to a lesser extent to tRNAs, could lead to decreased protein synthesis. BioMed Central 2012-07-10 /pmc/articles/PMC3393617/ /pubmed/22672539 http://dx.doi.org/10.1186/1743-422X-9-103 Text en Copyright ©2012 Abbas et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Abbas, Wasim
Dichamp, Isabelle
Herbein, Georges
The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA
title The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA
title_full The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA
title_fullStr The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA
title_full_unstemmed The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA
title_short The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA
title_sort hiv-1 nef protein interacts with two components of the 40s small ribosomal subunit, the rps10 protein and the 18s rrna
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3393617/
https://www.ncbi.nlm.nih.gov/pubmed/22672539
http://dx.doi.org/10.1186/1743-422X-9-103
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