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siRNA against the G gene of human metapneumovirus

BACKGROUND: Human metapneumovirus (hMPV) is a significant viral respiratory pathogen of infants and children, the elderly and immunocompromised individuals. Disease associated with hMPV infection resembles that of human respiratory syncytial virus (RSV) and includes bronchiolitis and pneumonia. The...

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Autores principales: Preston, Faith Maxine, Straub, Claire P, Ramirez, Ruben, Mahalingam, Suresh, Spann, Kirsten M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3393630/
https://www.ncbi.nlm.nih.gov/pubmed/22676157
http://dx.doi.org/10.1186/1743-422X-9-105
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author Preston, Faith Maxine
Straub, Claire P
Ramirez, Ruben
Mahalingam, Suresh
Spann, Kirsten M
author_facet Preston, Faith Maxine
Straub, Claire P
Ramirez, Ruben
Mahalingam, Suresh
Spann, Kirsten M
author_sort Preston, Faith Maxine
collection PubMed
description BACKGROUND: Human metapneumovirus (hMPV) is a significant viral respiratory pathogen of infants and children, the elderly and immunocompromised individuals. Disease associated with hMPV infection resembles that of human respiratory syncytial virus (RSV) and includes bronchiolitis and pneumonia. The glycosylated G attachment protein of hMPV is required for viral entry in vivo and has also been identified as an inhibitor of innate immune responses. FINDINGS: We designed and validated two siRNA molecules against the G gene using A549 cells and demonstrated consistent 88-92% knock-down for one siRNA molecule, which was used in subsequent experiments. Significant reduction of G mRNA in A549 cells infected with hMPV did not result in a reduction in viral growth, nor did it significantly increase the production of type I interferon (α/β) in response to infection. However, there was a moderate increase in IFN-β mRNA expression in response to infection in siG-transfected cells compared to untransfected and si-mismatch-transfected cells. Expression of G by recombinant adenovirus did not affect type I IFN expression. CONCLUSION: G has been previously described as a type I interferon antagonist, although our findings suggest it may not be a significant antagonist.
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spelling pubmed-33936302012-07-11 siRNA against the G gene of human metapneumovirus Preston, Faith Maxine Straub, Claire P Ramirez, Ruben Mahalingam, Suresh Spann, Kirsten M Virol J Short Report BACKGROUND: Human metapneumovirus (hMPV) is a significant viral respiratory pathogen of infants and children, the elderly and immunocompromised individuals. Disease associated with hMPV infection resembles that of human respiratory syncytial virus (RSV) and includes bronchiolitis and pneumonia. The glycosylated G attachment protein of hMPV is required for viral entry in vivo and has also been identified as an inhibitor of innate immune responses. FINDINGS: We designed and validated two siRNA molecules against the G gene using A549 cells and demonstrated consistent 88-92% knock-down for one siRNA molecule, which was used in subsequent experiments. Significant reduction of G mRNA in A549 cells infected with hMPV did not result in a reduction in viral growth, nor did it significantly increase the production of type I interferon (α/β) in response to infection. However, there was a moderate increase in IFN-β mRNA expression in response to infection in siG-transfected cells compared to untransfected and si-mismatch-transfected cells. Expression of G by recombinant adenovirus did not affect type I IFN expression. CONCLUSION: G has been previously described as a type I interferon antagonist, although our findings suggest it may not be a significant antagonist. BioMed Central 2012-07-10 /pmc/articles/PMC3393630/ /pubmed/22676157 http://dx.doi.org/10.1186/1743-422X-9-105 Text en Copyright ©2012 Preston et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Preston, Faith Maxine
Straub, Claire P
Ramirez, Ruben
Mahalingam, Suresh
Spann, Kirsten M
siRNA against the G gene of human metapneumovirus
title siRNA against the G gene of human metapneumovirus
title_full siRNA against the G gene of human metapneumovirus
title_fullStr siRNA against the G gene of human metapneumovirus
title_full_unstemmed siRNA against the G gene of human metapneumovirus
title_short siRNA against the G gene of human metapneumovirus
title_sort sirna against the g gene of human metapneumovirus
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3393630/
https://www.ncbi.nlm.nih.gov/pubmed/22676157
http://dx.doi.org/10.1186/1743-422X-9-105
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