Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach

The diffusion coefficient of fluorescein in detached cilia of Xenopus laevis olfactory receptor neurons was measured using spatially-resolved FRAP, where the dye along half of the ciliary length was photobleached and its spatiotemporal fluorescence redistribution recorded. Fitting a one-dimensional...

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Detalles Bibliográficos
Autores principales: Alevra, Mihai, Schwartz, Peter, Schild, Detlev
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3393724/
https://www.ncbi.nlm.nih.gov/pubmed/22808046
http://dx.doi.org/10.1371/journal.pone.0039628
Descripción
Sumario:The diffusion coefficient of fluorescein in detached cilia of Xenopus laevis olfactory receptor neurons was measured using spatially-resolved FRAP, where the dye along half of the ciliary length was photobleached and its spatiotemporal fluorescence redistribution recorded. Fitting a one-dimensional numerical simulation of diffusion and photobleaching for 35 cilia resulted in a mean value of the diffusion coefficient [Image: see text] and thus a reduction by a factor of [Image: see text] compared to free diffusion in aqueous solution.

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