Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach

The diffusion coefficient of fluorescein in detached cilia of Xenopus laevis olfactory receptor neurons was measured using spatially-resolved FRAP, where the dye along half of the ciliary length was photobleached and its spatiotemporal fluorescence redistribution recorded. Fitting a one-dimensional...

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Detalles Bibliográficos
Autores principales: Alevra, Mihai, Schwartz, Peter, Schild, Detlev
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3393724/
https://www.ncbi.nlm.nih.gov/pubmed/22808046
http://dx.doi.org/10.1371/journal.pone.0039628
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author Alevra, Mihai
Schwartz, Peter
Schild, Detlev
author_facet Alevra, Mihai
Schwartz, Peter
Schild, Detlev
author_sort Alevra, Mihai
collection PubMed
description The diffusion coefficient of fluorescein in detached cilia of Xenopus laevis olfactory receptor neurons was measured using spatially-resolved FRAP, where the dye along half of the ciliary length was photobleached and its spatiotemporal fluorescence redistribution recorded. Fitting a one-dimensional numerical simulation of diffusion and photobleaching for 35 cilia resulted in a mean value of the diffusion coefficient [Image: see text] and thus a reduction by a factor of [Image: see text] compared to free diffusion in aqueous solution.
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spelling pubmed-33937242012-07-17 Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach Alevra, Mihai Schwartz, Peter Schild, Detlev PLoS One Research Article The diffusion coefficient of fluorescein in detached cilia of Xenopus laevis olfactory receptor neurons was measured using spatially-resolved FRAP, where the dye along half of the ciliary length was photobleached and its spatiotemporal fluorescence redistribution recorded. Fitting a one-dimensional numerical simulation of diffusion and photobleaching for 35 cilia resulted in a mean value of the diffusion coefficient [Image: see text] and thus a reduction by a factor of [Image: see text] compared to free diffusion in aqueous solution. Public Library of Science 2012-07-10 /pmc/articles/PMC3393724/ /pubmed/22808046 http://dx.doi.org/10.1371/journal.pone.0039628 Text en Alevra et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Alevra, Mihai
Schwartz, Peter
Schild, Detlev
Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach
title Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach
title_full Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach
title_fullStr Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach
title_full_unstemmed Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach
title_short Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach
title_sort direct measurement of diffusion in olfactory cilia using a modified frap approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3393724/
https://www.ncbi.nlm.nih.gov/pubmed/22808046
http://dx.doi.org/10.1371/journal.pone.0039628
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