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MFEprimer-2.0: a fast thermodynamics-based program for checking PCR primer specificity

Evaluating the specificity of polymerase chain reaction (PCR) primers is an essential step in PCR primer design. The MFEprimer-2.0 server allows users to check primer specificity against genomic DNA and messenger RNA/complementary DNA sequence databases quickly and easily. MFEprimer-2.0 uses a k-mer...

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Detalles Bibliográficos
Autores principales:	Qu, Wubin, Zhou, Yang, Zhang, Yanchun, Lu, Yiming, Wang, Xiaolei, Zhao, Dongsheng, Yang, Yi, Zhang, Chenggang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2012
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394324/ https://www.ncbi.nlm.nih.gov/pubmed/22689644 http://dx.doi.org/10.1093/nar/gks552

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