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Optical imaging to trace near infrared fluorescent zinc oxide nanoparticles following oral exposure

BACKGROUND: Understanding how nanomaterials are distributed in the body after exposure is important for assessing whether they are safe. In this study, we investigated the behavior and accumulation of nanoscaled and submicron-scaled zinc oxide (ZnO) particles in the body using optical imaging follow...

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Autores principales: Lee, Chang-Moon, Jeong, Hwan-Jeong, Yun, Kuk-No, Kim, Dong Wook, Sohn, Myung-Hee, Lee, Jong Kwon, Jeong, Jayoung, Lim, Seok Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394462/
https://www.ncbi.nlm.nih.gov/pubmed/22811605
http://dx.doi.org/10.2147/IJN.S32828
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author Lee, Chang-Moon
Jeong, Hwan-Jeong
Yun, Kuk-No
Kim, Dong Wook
Sohn, Myung-Hee
Lee, Jong Kwon
Jeong, Jayoung
Lim, Seok Tae
author_facet Lee, Chang-Moon
Jeong, Hwan-Jeong
Yun, Kuk-No
Kim, Dong Wook
Sohn, Myung-Hee
Lee, Jong Kwon
Jeong, Jayoung
Lim, Seok Tae
author_sort Lee, Chang-Moon
collection PubMed
description BACKGROUND: Understanding how nanomaterials are distributed in the body after exposure is important for assessing whether they are safe. In this study, we investigated the behavior and accumulation of nanoscaled and submicron-scaled zinc oxide (ZnO) particles in the body using optical imaging following oral exposure. METHODS: To trace these nanoparticles in the body, ZnO nanoparticles were conjugated with a monoreactive hydroxysuccinimide ester of Cy5.5 (Cy5.5-NHS), and the conjugation-stabilizing effect of Cy5.5 on the nanoparticles was evaluated in simulated gastric fluid (pH 1.2) for 7 hours. To compare the distribution of Cy5.5-NHS and Cy5.5-conjugated ZnO nanoparticles, Cy5.5-NHS 0.5 mg/kg and Cy5.5-conjugated ZnO nanoparticles 250 mg/kg were administered orally to healthy rats. We collected blood from the rats at predesignated time points for 7 hours after administration, and optical imaging studies were performed at 1, 2, 3, 5, and 7 hours after dosing. To investigate the extent of nanoparticle accumulation in the organs and tissues, the mice were sacrificed at 23 hours after administration, and the organs were removed and imaged. RESULTS: Cy5.5-conjugated ZnO nanoparticles were stable in simulated gastric fluid for 7 hours. The signal intensity of Cy5.5-NHS in blood was highest 3 hours after oral administration, and Cy5.5-conjugated ZnO nanoparticles showed the highest signal intensity in blood 5–7 hours after administration. In vivo optical images indicated that Cy5.5-NHS showed optical signals in the lung, liver, and gastrointestinal tract after oral administration, whereas Cy5.5-conjugated ZnO nanoparticles were seen only in the gastrointestinal tract. Seven hours following administration, biodistribution studies demonstrated that Cy5.5-NHS accumulated in the lung and liver, and Cy5.5-conjugated ZnO nanoparticles resulted in a strong signal in the kidney and liver. Different-sized ZnO nanoparticles showed dissimilar patterns of biodistribution in ex vivo optical images. CONCLUSION: ZnO nanoparticles are absorbed into the tissues following oral exposure and their behavior can be monitored and evaluated using optical imaging.
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spelling pubmed-33944622012-07-18 Optical imaging to trace near infrared fluorescent zinc oxide nanoparticles following oral exposure Lee, Chang-Moon Jeong, Hwan-Jeong Yun, Kuk-No Kim, Dong Wook Sohn, Myung-Hee Lee, Jong Kwon Jeong, Jayoung Lim, Seok Tae Int J Nanomedicine Original Research BACKGROUND: Understanding how nanomaterials are distributed in the body after exposure is important for assessing whether they are safe. In this study, we investigated the behavior and accumulation of nanoscaled and submicron-scaled zinc oxide (ZnO) particles in the body using optical imaging following oral exposure. METHODS: To trace these nanoparticles in the body, ZnO nanoparticles were conjugated with a monoreactive hydroxysuccinimide ester of Cy5.5 (Cy5.5-NHS), and the conjugation-stabilizing effect of Cy5.5 on the nanoparticles was evaluated in simulated gastric fluid (pH 1.2) for 7 hours. To compare the distribution of Cy5.5-NHS and Cy5.5-conjugated ZnO nanoparticles, Cy5.5-NHS 0.5 mg/kg and Cy5.5-conjugated ZnO nanoparticles 250 mg/kg were administered orally to healthy rats. We collected blood from the rats at predesignated time points for 7 hours after administration, and optical imaging studies were performed at 1, 2, 3, 5, and 7 hours after dosing. To investigate the extent of nanoparticle accumulation in the organs and tissues, the mice were sacrificed at 23 hours after administration, and the organs were removed and imaged. RESULTS: Cy5.5-conjugated ZnO nanoparticles were stable in simulated gastric fluid for 7 hours. The signal intensity of Cy5.5-NHS in blood was highest 3 hours after oral administration, and Cy5.5-conjugated ZnO nanoparticles showed the highest signal intensity in blood 5–7 hours after administration. In vivo optical images indicated that Cy5.5-NHS showed optical signals in the lung, liver, and gastrointestinal tract after oral administration, whereas Cy5.5-conjugated ZnO nanoparticles were seen only in the gastrointestinal tract. Seven hours following administration, biodistribution studies demonstrated that Cy5.5-NHS accumulated in the lung and liver, and Cy5.5-conjugated ZnO nanoparticles resulted in a strong signal in the kidney and liver. Different-sized ZnO nanoparticles showed dissimilar patterns of biodistribution in ex vivo optical images. CONCLUSION: ZnO nanoparticles are absorbed into the tissues following oral exposure and their behavior can be monitored and evaluated using optical imaging. Dove Medical Press 2012 2012-06-27 /pmc/articles/PMC3394462/ /pubmed/22811605 http://dx.doi.org/10.2147/IJN.S32828 Text en © 2012 Lee et al publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Lee, Chang-Moon
Jeong, Hwan-Jeong
Yun, Kuk-No
Kim, Dong Wook
Sohn, Myung-Hee
Lee, Jong Kwon
Jeong, Jayoung
Lim, Seok Tae
Optical imaging to trace near infrared fluorescent zinc oxide nanoparticles following oral exposure
title Optical imaging to trace near infrared fluorescent zinc oxide nanoparticles following oral exposure
title_full Optical imaging to trace near infrared fluorescent zinc oxide nanoparticles following oral exposure
title_fullStr Optical imaging to trace near infrared fluorescent zinc oxide nanoparticles following oral exposure
title_full_unstemmed Optical imaging to trace near infrared fluorescent zinc oxide nanoparticles following oral exposure
title_short Optical imaging to trace near infrared fluorescent zinc oxide nanoparticles following oral exposure
title_sort optical imaging to trace near infrared fluorescent zinc oxide nanoparticles following oral exposure
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394462/
https://www.ncbi.nlm.nih.gov/pubmed/22811605
http://dx.doi.org/10.2147/IJN.S32828
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