Cargando…
IL-17A Facilitates Platelet Function through the ERK2 Signaling Pathway in Patients with Acute Coronary Syndrome
BACKGROUND: Platelet aggregation mediated by inflammation played a critical role in the development of coronary heart diseases (CHD). Our previous clinical researches showed that Th17 cells and their characteristic cytokine IL-17A were associated with the plaque destabilization in patients with acut...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394751/ https://www.ncbi.nlm.nih.gov/pubmed/22808218 http://dx.doi.org/10.1371/journal.pone.0040641 |
_version_ | 1782237890397339648 |
---|---|
author | Zhang, Shuang Yuan, Jing Yu, Miao Fan, Hong Guo, Zhang-Qiang Yang, Rui Guo, He-Ping Liao, Yu-Hua Wang, Min |
author_facet | Zhang, Shuang Yuan, Jing Yu, Miao Fan, Hong Guo, Zhang-Qiang Yang, Rui Guo, He-Ping Liao, Yu-Hua Wang, Min |
author_sort | Zhang, Shuang |
collection | PubMed |
description | BACKGROUND: Platelet aggregation mediated by inflammation played a critical role in the development of coronary heart diseases (CHD). Our previous clinical researches showed that Th17 cells and their characteristic cytokine IL-17A were associated with the plaque destabilization in patients with acute coronary syndrome (ACS). However, the potent effect of IL-17A on platelets-induced atherothrombosis remains unknown. METHODS AND RESULTS: In this study, we detected the plasma IL-17A levels and platelet aggregation in patients with stable angina (SA), unstable angina (UA), acute myocardial infarction (AMI) and chest pain syndrome (CPS). In addition, the markers of platelet activation (CD62P/PAC-1) and the mitogen-activated protein kinases (MAPKs) pathway were detected in platelets from ACS patients. We found that plasma IL-17A levels and platelet aggregation in patients with ACS (UA and AMI) were significantly higher than patients with SA and CPS, and the plasma IL-17A levels were positively correlated with the platelet aggregation (R = 0.47, P<0.01). In addition, in patients with ACS, the platelet aggregation, CD62P/PAC-1 and the phosphorylation of ERK2 signaling pathway were obviously elevated in platelets pre-stimulated with IL-17A in vitro. Furthermore, the specific inhibitor of ERK2 could attenuate platelet aggregation and activation triggered by IL-17A. CONCLUSION: Our experiment firstly proved that IL-17A could promote platelet function in patients with ACS via activating platelets ERK2 signaling pathway and may provide a novel target for antiplatelet therapies in CHD. |
format | Online Article Text |
id | pubmed-3394751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33947512012-07-17 IL-17A Facilitates Platelet Function through the ERK2 Signaling Pathway in Patients with Acute Coronary Syndrome Zhang, Shuang Yuan, Jing Yu, Miao Fan, Hong Guo, Zhang-Qiang Yang, Rui Guo, He-Ping Liao, Yu-Hua Wang, Min PLoS One Research Article BACKGROUND: Platelet aggregation mediated by inflammation played a critical role in the development of coronary heart diseases (CHD). Our previous clinical researches showed that Th17 cells and their characteristic cytokine IL-17A were associated with the plaque destabilization in patients with acute coronary syndrome (ACS). However, the potent effect of IL-17A on platelets-induced atherothrombosis remains unknown. METHODS AND RESULTS: In this study, we detected the plasma IL-17A levels and platelet aggregation in patients with stable angina (SA), unstable angina (UA), acute myocardial infarction (AMI) and chest pain syndrome (CPS). In addition, the markers of platelet activation (CD62P/PAC-1) and the mitogen-activated protein kinases (MAPKs) pathway were detected in platelets from ACS patients. We found that plasma IL-17A levels and platelet aggregation in patients with ACS (UA and AMI) were significantly higher than patients with SA and CPS, and the plasma IL-17A levels were positively correlated with the platelet aggregation (R = 0.47, P<0.01). In addition, in patients with ACS, the platelet aggregation, CD62P/PAC-1 and the phosphorylation of ERK2 signaling pathway were obviously elevated in platelets pre-stimulated with IL-17A in vitro. Furthermore, the specific inhibitor of ERK2 could attenuate platelet aggregation and activation triggered by IL-17A. CONCLUSION: Our experiment firstly proved that IL-17A could promote platelet function in patients with ACS via activating platelets ERK2 signaling pathway and may provide a novel target for antiplatelet therapies in CHD. Public Library of Science 2012-07-11 /pmc/articles/PMC3394751/ /pubmed/22808218 http://dx.doi.org/10.1371/journal.pone.0040641 Text en Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhang, Shuang Yuan, Jing Yu, Miao Fan, Hong Guo, Zhang-Qiang Yang, Rui Guo, He-Ping Liao, Yu-Hua Wang, Min IL-17A Facilitates Platelet Function through the ERK2 Signaling Pathway in Patients with Acute Coronary Syndrome |
title | IL-17A Facilitates Platelet Function through the ERK2 Signaling Pathway in Patients with Acute Coronary Syndrome |
title_full | IL-17A Facilitates Platelet Function through the ERK2 Signaling Pathway in Patients with Acute Coronary Syndrome |
title_fullStr | IL-17A Facilitates Platelet Function through the ERK2 Signaling Pathway in Patients with Acute Coronary Syndrome |
title_full_unstemmed | IL-17A Facilitates Platelet Function through the ERK2 Signaling Pathway in Patients with Acute Coronary Syndrome |
title_short | IL-17A Facilitates Platelet Function through the ERK2 Signaling Pathway in Patients with Acute Coronary Syndrome |
title_sort | il-17a facilitates platelet function through the erk2 signaling pathway in patients with acute coronary syndrome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394751/ https://www.ncbi.nlm.nih.gov/pubmed/22808218 http://dx.doi.org/10.1371/journal.pone.0040641 |
work_keys_str_mv | AT zhangshuang il17afacilitatesplateletfunctionthroughtheerk2signalingpathwayinpatientswithacutecoronarysyndrome AT yuanjing il17afacilitatesplateletfunctionthroughtheerk2signalingpathwayinpatientswithacutecoronarysyndrome AT yumiao il17afacilitatesplateletfunctionthroughtheerk2signalingpathwayinpatientswithacutecoronarysyndrome AT fanhong il17afacilitatesplateletfunctionthroughtheerk2signalingpathwayinpatientswithacutecoronarysyndrome AT guozhangqiang il17afacilitatesplateletfunctionthroughtheerk2signalingpathwayinpatientswithacutecoronarysyndrome AT yangrui il17afacilitatesplateletfunctionthroughtheerk2signalingpathwayinpatientswithacutecoronarysyndrome AT guoheping il17afacilitatesplateletfunctionthroughtheerk2signalingpathwayinpatientswithacutecoronarysyndrome AT liaoyuhua il17afacilitatesplateletfunctionthroughtheerk2signalingpathwayinpatientswithacutecoronarysyndrome AT wangmin il17afacilitatesplateletfunctionthroughtheerk2signalingpathwayinpatientswithacutecoronarysyndrome |